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First exams 2025

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Stages of Photosynthesis (CIE A Level Biology)

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The Two Stages of Photosynthesis

  • Photosynthesis occurs in two distinct stages:
    • The light-dependent stage, which takes place in the thylakoids
    • The light-independent stage, which takes place in the stroma

Summary of the light-dependent stage 

  • Reduced NADP is produced when hydrogen ions combine with the carrier molecule NADP using electrons from the photolysis of water
  • ATP is produced  in a process called photophosphorylation 
    • Photophosphorylation uses the proton (H+) gradient generated by the photolysis of water
  • Energy from ATP and hydrogen from reduced NADP are passed from the light-dependent stage to the light-independent stage of photosynthesis

Summary of the light-independent stage

  • The energy and hydrogen (from the light-dependent stage) are used
  • This takes place in reactions known collectively as the Calvin cycle
  • Complex organic molecules are produced, including (but not limited to) carbohydrates, such as:
    • Starch (for storage)
    • Sucrose (for translocation around the plant)
    • Cellulose (for making cell walls)

Photosynthesis Stages - Overview Diagram

The two stages of photosynthesis

Photosynthesis occurs in two, closely-linked stages: the light-dependent stage, which takes place in the thylakoids, and the light-independent stage, which takes place in the stroma

Exam Tip

Remember, the whole purpose of the light-dependent reactions is to produce ATP and reduced NADP, which are then supplied into the stroma and used to complete the process of photosynthesis through the light-independent reactions.

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Phil

Author: Phil

Phil has a BSc in Biochemistry from the University of Birmingham, followed by an MBA from Manchester Business School. He has 15 years of teaching and tutoring experience, teaching Biology in schools before becoming director of a growing tuition agency. He has also examined Biology for one of the leading UK exam boards. Phil has a particular passion for empowering students to overcome their fear of numbers in a scientific context.