Limiting Factors (Cambridge O Level Biology)

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Limiting Factors

  • A plant that is given unlimited sunlight, carbon dioxide and water, and that is in a warm environment, will be able to photosynthesise at its maximum rate
  • However, most plants do not have unlimited supplies of their raw materials, so their rate of photosynthesis is limited by whichever factor is in shortest supply at any given time
  • So, a limiting factor can be defined as:

An environmental factor that limits the rate of a reaction

  • There are three main factors which limit the rate of photosynthesis: 
    • Temperature 
    • Light intensity
    • Carbon dioxide concentration
  • Note that although water is necessary for photosynthesis, it is not considered a limiting factor
    • Water is involved in multiple plant processes, such as transport and cell turgidity, so it is likely to affect other plant processes before it directly affects the rate of photosynthesis, e.g. limited water availability will cause stomata to close, limiting carbon dioxide availability

Temperature

  • As temperature increases, the rate of photosynthesis increases
    • On a graph of temperature against rate of photosynthesis, this can be seen as an upward curve
  • This trend continues up to a temperature at which the enzymes begin to denature and the rate of reaction then decreases

Temperature and photosynthesis graph

The effect of temperature on the rate of photosynthesis graph

As temperature increases, the rate of photosynthesis increases. The continues up to a temperature at which enzyme denaturation occurs, after which the rate of photosynthesis decreases

Light intensity

  • The higher the light intensity, the faster the rate of photosynthesis
    • On a graph of light intensity and rate of photosynthesis, this can be seen as a linear relationship
    • While the graph shows this linear relationship, light intensity is the limiting factor
  • This trend will continue until some other factor required for photosynthesis prevents the rate from increasing further
    • At this point, the factor that prevents the rate from increasing further becomes the limiting factor; the new limiting factor could be:
      • Temperature
      • Carbon dioxide concentration

Light intensity and photosynthesis graph

The effect of light intensity on the rate of photosynthesis graph

As light intensity increases, so does the rate of photosynthesis; this can be seen as a linear relationship on a graph. Once the graph levels off, another factor, e.g. temperature, has become the limiting factor

Carbon dioxide concentration

  • As carbon dioxide concentration increases, the rate of photosynthesis increases
    • On a graph of carbon dioxide concentration and rate of photosynthesis, this can be seen as a linear relationship
    • While the graph shows this linear relationship, carbon dioxide concentration is the limiting factor
  • This trend will continue until some other factor required for photosynthesis prevents the rate from increasing further; the new limiting factor could be:
    • Temperature
    • Light intensity

Carbon dioxide concentration and photosynthesis graph

The effect of carbon dioxide concentration on the rate of photosynthesis graph

As carbon dioxide concentration increases, so does the rate of photosynthesis. This continues until another factors becomes limiting

Examiner Tip

Interpreting graphs of limiting factors can be confusing for many students, so remember the following:

  • In the section of the graph where the rate is increasing (the line is going up), the limiting factor matches the label on the x axis of the graph
  • In the section of the graph where the rate is not increasing (the line is horizontal), the limiting factor will be something other than what is on the x axis – choose from temperature, light intensity or carbon dioxide concentration

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Naomi H

Author: Naomi H

Expertise: Biology

Naomi graduated from the University of Oxford with a degree in Biological Sciences. She has 8 years of classroom experience teaching Key Stage 3 up to A-Level biology, and is currently a tutor and A-Level examiner. Naomi especially enjoys creating resources that enable students to build a solid understanding of subject content, while also connecting their knowledge with biology’s exciting, real-world applications.