Enzymes - Roles & Modes of Action (Edexcel International AS Biology)

• Enzymes are globular proteins
• This means their 3D shape (as well as the shape of the active site of an enzyme) is determined by the complex tertiary structure of the protein that makes up the enzyme and is therefore highly specific
• Enzymes have a unique active site where specific substrates bind forming an enzyme-substrate complex
• The active site of an enzyme has a specific shape to fit a specific substrate
• Extremes of heat or pH can alter the protein structure and change the shape of the active site, preventing substrate binding – this is called denaturation
• Substrates collide with the enzymes active site and this must happen at the correct orientation and speed in order for a reaction to occur

The active site of an enzyme has a specific shape to fit a specific substrate (when the substrate binds an enzyme-substrate complex is formed)

Enzyme specificity

• The specificity of an enzyme is a result of the complementary nature between the shape of the active site on the enzyme and its substrate(s)
• Only one specific substrate will fit into one specific active site
• The shape of the active site (and therefore the specificity of the enzyme) is determined by the complex tertiary structure of the protein that makes up the enzyme:
  • Proteins are formed from chains of amino acids held together by peptide bonds
  • The order of amino acids determines the shape of an enzyme
  • If the order is altered, the resulting three-dimensional shape changes
• If the tertiary structure of the protein is altered in any way, the shape of the active site will change and the substrate will no longer fit the active site
• This means that an enzyme-substrate complex will not be able to form and the product(s) will not be produced: the enzyme will not be able to carry out its function

An example of enzyme specificity

The lock-and-key hypothesis

• In the 1890’s the first model of enzyme activity was described by Emil Fischer:
  • He suggested that both enzymes and substrates were rigid structures that locked into each other very precisely, much like a key going into a lock
  • This is known as the ‘lock-and-key hypothesis’

The Lock and Key hypothesis

The induced-fit hypothesis

• The lock-and-key model was later modified and adapted to our current understanding of enzyme activity, permitted by advances in techniques in the molecular sciences
• The modified model of enzyme activity (first proposed in 1959) is known as the ‘induced-fit hypothesis’
• Although it is very similar to the lock and key hypothesis, in this model the enzyme and substrate interact with each other:
  • The enzyme and its active site (and sometimes the substrate) can change shape slightly as the substrate molecule enters the enzyme
  • These changes in shape are known as conformational changes
  • The conformational changes ensure an ideal binding arrangement between the enzyme and substrate is achieved
  • This maximises the ability of the enzyme to catalyse the reaction

The Induced Fit model of enzyme action

• Enzymes are biological catalysts
  • ‘Biological’ because they function in living systems
  • ‘Catalysts’ because they speed up the rate of chemical reactions without being used up or undergoing permanent change
  • They speed up reactions by reducing the activation energy of reactions

Enzymes and the lowering of activation energy

• All chemical reactions are associated with energy changes
• For a reaction to proceed there must be enough activation energy
• Activation energy is the amount of energy needed by the substrate to become just unstable enough for a reaction to occur and for products to be formed
  • Enzymes speed up chemical reactions because they reduce the stability of bonds in the reactants
  • The destabilisation of bonds in the substrate makes it more reactive
• Rather than lowering the overall energy change of the reaction, enzymes work by providing an alternative energy pathway with a lower activation energy
• Without enzymes, extremely high temperatures or pressures would be needed to reach the activation energy for many biological reactions
  • Enzymes avoid the need for these extreme conditions (that would otherwise kill cells)

The activation energy of a chemical reaction is lowered by the presence of a catalyst (i.e. an enzyme)

• Enzymes are globular proteins with complex tertiary structures
  • Some are formed from a single polypeptide, whilst others are made up of two or more polypeptides and therefore have a quaternary structure
• Metabolic pathways are controlled by enzymes in a biochemical cascade of reactions
  • Metabolism is a combination of anabolic and catabolic reactions
    • New molecules are built up during anabolic reactions
    • Large, complex molecules are broken down into smaller, simpler ones during catabolic reactions 
  • Virtually every metabolic reaction within living organisms is catalysed by an enzyme
  • Enzymes are therefore essential for life to exist
• All enzymes are proteins that are produced via the process of protein synthesis inside cells
• Some enzymes remain inside cells, whilst others are secreted to work outside of cells
• Enzymes can be intracellular or extracellular referring to whether they are active inside or outside the cell respectively
  • Intracellular enzymes are produced and function inside the cell
  • Extracellular enzymes are secreted by cells and catalyse reactions outside cells (eg. digestive enzymes in the gut)

Intracellular & Extracellular Enzymes Table