Genetic Modification (Genetic Engineering) (Edexcel IGCSE Biology: Double Science)

Scientists have developed genetically modified (GM) crops in order to increase food production by increasing crop yields.

Some GM crops are described as transgenic.

(2)

Give the role of two named enzymes in the production of GM organisms.

(2)

Some GM crops that are available to farmers are resistant to herbicides (weedkillers).

Other GM crops are resistant to diseases caused by viruses and to damage by insects.

Some people support the use of GM crops because they may be beneficial to ecosystems.

Some people oppose the use of GM crops because they could harm ecosystems.

Discuss these opinions for and against the use of GM crops.

The diagram shows the process of genetic modification used to produce multiple copies of a protein from a desired gene.

Identify structures W-Z in the diagram using the words below. Note that more words have been provided than are needed.

Recombinant DNA     DNA Ligase      Transgenic organism     

Bacterial plasmid      Desired gene      Insulin protein      

Identify the vector used in this genetic modification process.

The enzyme used at both W and X in the diagram in part (a) is a restriction enzyme.

Describe the role of the restriction enzyme at W and X.

Describe what needs to happen to structure Z in part (a) in order to produce many copies of the protein coded for by the desired gene.

The process of genetic modification involves the use of enzymes that cut DNA.

Name the enzymes that are used to cut DNA.

(1)

Explain why the enzymes named in part (i) are so useful in genetic modification.

(2)

Genes can be extracted from other species and inserted into crop plants. For example, a gene that codes for the bacterial toxin known as Bt provides resistance to insect pests in crops.

Other than pest resistance, give two reasons for inserting genes from other species into crop plants.

(2)

(ii)

Suggest why inserting DNA into plant cells can be a challenge.

(1)

Some environmentalists are concerned about inserting Bt toxin genes into crop plants.

Suggest why some are concerned about the insertion of genes from other species, such as the gene for Bt toxin, into crop plants.

Plants can be genetically modified (GM) to produce insect poison.

They are modified using a bacterium called Agrobacterium.

This bacterium has a plasmid that contains recombinant DNA.

Describe how the plasmid is modified to contain recombinant DNA.

A farmer can use either of these methods to improve his crop yield.

• Grow GM plants that produce the insect poison
• Grow non-GM plants and use pesticides

The farmer decides to grow the GM plants rather than using pesticides.

Discuss the decision made by the farmer.

Insulin is a hormone that is used to treat people with type 1 diabetes. 

Describe the function of insulin in the body.

The insulin used by diabetic patients is produced by genetically modified bacteria. 

Describe the process used to create bacteria that contain the gene for human insulin. 

Before the process in part (b) was developed, insulin for the treatment of diabetes could only be obtained from sheep and pigs.

Suggest two reasons, other than preventing the exploitation of animals, why it is better to obtain insulin by genetic engineering than from animals.

After the bacteria have been genetically modified they are placed in a machine called a fermenter where they can reproduce asexually and increase the number of genetically modified bacteria in the culture. 

Bacteria reproduce by dividing into two identical cells. The species of bacteria used in this process has a division time of 30 minutes.  

One genetically modified bacterial cell is added to a fermenter.

Calculate the number of bacteria that will be present in a fermenter after four and a half hours.

(3)

Describe how conditions inside the fermenter could be controlled to maximise the reproduction of genetically modified bacteria.

(3)

As part of a global scientific push to improve the efficiency and sustainability of agriculture, scientists have developed a transgenic pig. The process used to create the transgenic pig, known as 'Enviropig', is summarised below.

1. Genes are taken from E. coli bacteria and also from mice.
2. The combined E. coli and mouse genes are inserted into a fertilised pig egg cell nucleus using a microscope and a very small, sharp, pipette.
3. The newly modified egg cell divides and develops into an embryo.
4. The embryo is implanted into the uterus of a surrogate pig and allowed to develop to maturity.
5. The resulting piglet is a transgenic 'Enviropig' that is able to produce E. coli and mouse proteins.

Explain why a plasmid vector is not a suitable way of inserting the desired genes into the fertilised pig egg cell in step 2.

(2)

Explain why all of the cells in the pig embryo that develops in step 3 contain the new genes.

(2)

Separate: Biology Only

Use your knowledge of the role of DNA to explain why the piglet in step 5 is able to produce E. coli and mouse proteins.

(2)

The genes inserted into the genome of 'Enviropig' allow pigs to produce an enzyme called phytase in their saliva. Phytase enables the pigs to digest phosphorus, a mineral that is required in the diet of pigs, but that would otherwise be egested by pigs in their faeces.

Pigs that are unable to produce their own phytase need to take phytase supplements in their food, and still egest a large amount of phosphorus in their waste.

Note that phosphorus is a mineral often found in industrial fertilisers.

Use the information provided and your own knowledge to discuss the potential benefits of 'Enviropig'.

The graph below shows the phosporus levels in the faeces of pigs with different percentages of soybean meal in their diet.

Calculate the percentage decrease in faecal phosphorus between non-transgenic and transgenic pigs on a diet of 54 % soybean meal.

(2)

An environmental science student read the results of the study shown in part (c) and reached the following conclusion:

"In the future, 'Enviropigs' will provide a solution to the sustainability issues around pig farming worldwide"

Evaluate the student's conclusion.