Food Tests (Cambridge (CIE) IGCSE Biology) : Revision Note
Learn all about food tests in biology for your IGCSE exam. Find information about food tests for glucose, starch, protein, lipids and vitamin C.
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Food tests in biology
Food test for glucose (a reducing sugar)
Add Benedict's solution into sample solution in test tube
Heat at 60 - 70 °c in water bath for 5 minutes
Take test tube out of water bath and observe the colour
A positive test will show a colour change from blue to orange or brick red

Food test for starch
We can use iodine to test for the presence or absence of starch in a food sample.

Add drops of iodine solution to the food sample
A positive test will show a colour change from orange-brown to blue-black

Food test for protein
Add drops of Biuret solution to the food sample
A positive test will show a colour change from blue to violet / purple

Food test for lipids
Food sample is mixed with 2cm3 of ethanol and shaken
The ethanol is added to an equal volume of cold water
A positive test will show a cloudy emulsion forming

Food test for vitamin C
Add 1cm3 of DCPIP solution to a test tube
Add a small amount of food sample (as a solution)
A positive test will show the blue colour of the dye disappearing

Examiner Tips and Tricks
When describing food tests in exam answers, make sure you give the starting colour of the solution and the colour it changes to for a positive result.
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