Microorganisms & Enzymes (AQA GCSE Food Preparation & Nutrition)

Revision Note

Naomi Holyoak

Written by: Naomi Holyoak

Reviewed by: Lára Marie McIvor

Microorganisms, enzymes & food

  • Food spoilage can be defined as:

Loss of food quality that causes food to become inedible

  • Food spoilage can occur due to:

    • activity of microorganisms

    • enzyme activity within the food

Microorganisms and food spoilage

  • Microorganisms are very small organisms that can only be seen using a microscope, e.g.

    • bacteria

    • fungi, such as yeast and mould

  • Not all microorganisms are harmful, but some can cause food spoilage and may result in food poisoning if consumed

Image shows diagrams of a bacterial cell, yeast cells, and mould growing on food.
Examples of microorganisms include bacteria, yeast and mould

Conditions for microorganism growth

  • In order to grow, microorganisms need specific conditions, including:

    • correct temperature

      • Conditions need to be warm enough for microorganisms to survive without being hot enough to destroy them

    • water

      • Moisture needs to be present in the food or in the surrounding air

    • food

      • Microorganisms need nutrients to enable them to survive and reproduce

    • correct pH

      • Most microorganisms cannot survive in highly acidic or highly alkaline conditions

  • In order to reach harmful levels, microorganisms need a sufficient length of time under suitable conditions

Preventing microorganism growth

  • Microorganism growth can be reduced by making the conditions inhospitable; this can be achieved by:

    • reducing the temperature, e.g. by putting food in the fridge or freezer

    • increasing the temperature by cooking food

    • reducing the length of time for which food is left at room temperature

    • providing an acidic environment, e.g. by pickling

    • removing water, e.g. by drying, salting or adding lots of sugar

A jar contains small cucumbers which are pickled in an acid
Pickling cucumbers in vinegar reduces the pH so that microorganisms cannot survive

Public domain, via pxhere

High risk foods for microorganism growth

  • Foods that spoil easily due to microorganism growth are known as high risk foods

    • These foods provide an environment that aids the growth of microorganisms; they are often

      • moist

      • high in protein, which provides food for microorganisms

    • High risk foods are generally 'ready-to-eat', meaning that they will not undergo a further heating process before consumption that might destroy microorganisms

  • Examples of high risk foods include:

    • cooked meat and fish

    • dairy products, such as cheese and milk

    • some forms of egg, and foods that contain egg, e.g. mayonnaise

    • precooked shellfish, e.g. mussels and prawns

    • meat products, including gravy, stock and sauces

    • cooked rice

Illustration of various foods: milk carton, cheese wedge, shrimp, salmon with vegetables, sausage, chicken legs, bowl of rice, gravy, eggs, and mayonnaise jar.
High risk foods are high in moisture and protein, e.g. cooked meat and fish, dairy and cooked rice

Enzymes and food spoilage

  • Enzymes are biological catalysts made of protein

  • The activity of enzymes that are already present inside foods can lead to enzymic browning

  • Food spoilage due to enzymes can be prevented by denaturing enzymes to reduce their activity; this can be achieved by:

    • heating, e.g. by blanching vegetables in boiling water for a few minutes before freezing

    • adding an acid, e.g. putting lemon juice on sliced apple

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Naomi Holyoak

Author: Naomi Holyoak

Expertise: Biology

Naomi graduated from the University of Oxford with a degree in Biological Sciences. She has 8 years of classroom experience teaching Key Stage 3 up to A-Level biology, and is currently a tutor and A-Level examiner. Naomi especially enjoys creating resources that enable students to build a solid understanding of subject content, while also connecting their knowledge with biology’s exciting, real-world applications.

Lára Marie McIvor

Author: Lára Marie McIvor

Expertise: Biology Lead

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.