Use of Chromatography in a Mixture of Dyes
Investigating a Mixture of Dyes
Practical Skills Development
- Safe use of a range of equipment to purify and/or separate chemical mixtures including chromatography
- Safe use and careful handling of liquids under controlled conditions
- Safe use of appropriate qualitative reagents and techniques to analyse and identify unknown samples or products
- Presenting observations and other data using appropriate formats
- The example given below focuses on chromatography, however you should be able to explain how other methods of separation including filtration, crystallisation, and distillation are used in a practical context
- Information on these methods can be located in 2.1 Separation Techniques
Aim
- To investigate how paper chromatography can be used to separate and identify a mixture of food colourings
Health & Safety Aspects
- No particular hazards unless organic solvents are used
Materials:
- A 250 cm3 beaker
- A wooden spill/splint
- A rectangle of chromatography paper
- Four known food colourings labelled A–D
- An unknown mixture of food colourings labelled U
- Five glass capillary tubes
- Paper clip
- Ruler & pencil
Diagram of the apparatus needed
Practical Tip:
The pencil line must never be below the level of the solvent as the samples will be washed away
Method:
- Use a ruler to draw a horizontal pencil line 2 cm from the end of the chromatography paper
- Use a different capillary tube to put a tiny spot of each colouring A, B, C and D on the line
- Use the fifth tube to put a small spot of the unknown mixture U on the line
- Make sure each spot is no more than 2-3 mm in diameter and label each spot in pencil
- Pour water into the beaker to a depth of no more than 1 cm and clip the top of the chromatography paper to the wooden spill. The top end is the furthest from the spots
- Carefully rest the wooden spill on the top edge of the beaker. The bottom edge of the paper should dip into the solvent
- Allow the solvent to travel undisturbed at least three quarters of the way up the paper
- Remove the paper and draw another pencil line on the dry part of the paper as close to the wet edge as possible. This is called the solvent front line
- Measure the distance in mm between the two pencil lines. This is the distance travelled by the water solvent
- For each of food colour A, B, C and D measure the distance in mm from the start line to the middle of the spot
Results
Conclusions
- The Rf values of food colours A, B, C and D should be compared to that for the unknown sample as well as a visual comparison being made
- The use of chromatography and Rf values is a viable method of identifying unknown mixtures given reference material