Photosynthesis (Edexcel GCSE Biology)

Exam Questions

3 hours18 questions
1a
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2 marks

State what is meant by the term photosynthesis.

1b
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2 marks

The graph below shows the relationship between carbon dioxide concentration and the rate of photosynthesis.

screenshot-2022-07-23-15-03-56

Describe the relationship between carbon dioxide concentration and the rate of photosynthesis shown in the graph.

1c
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1 mark

Identify a possible limiting factor at the point labelled X in the graph in part (b).

1d
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3 marks

Sketch a graph of the rate of photosynthesis against temperature.

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2a
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2 marks

Identify two limiting factors of photosynthesis.

2b
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1 mark

A student wanted to test the effect of light intensity on the number of bubbles produced by an aquatic plant over one hour.

Figure 1 shows how the student set up his investigation.

factors-that-affect-photosynthesis-1

Figure 1

Identify the gas being collected in the measuring cylinder which shows that the aquatic plant is photosynthesising.

2c
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1 mark

The student sets up the same experiment as shown in part (b) but this time, puts some ice cubes into the beaker.

State what effect this may have on the rate of photosynthesis of the plant.

2d
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1 mark

Water is usually not considered a limiting factor of photosynthesis.

Which statement explains why?

  A Global warming means there are lots of floods and so there is plenty of water.
  B If water availability is low, plants close their stomata to stop transpiration, meaning that photosynthesis is limited by carbon dioxide.
  C Water cannot be measured using a measuring cylinder.
  D Water is not needed for photosynthesis, only for respiration.

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3a
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1 mark

A student set up an investigation into the limiting factors of photosynthesis.

His set up is shown in Figure 1 below:

photosynthesis-prac-1

Figure 1

Identify the limiting factor being investigated in this practical.

3b
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2 marks

The student carried out an investigation to find out the effect of light intensity on the rate of photosynthesis by moving the light source further and further away from the aquatic plant and counting the bubbles produced per minute at each distance.

The results can be seen in the table below:

Distance between the aquatic plant and lamp in cm Number of bubbles counted in 1 minute
0 29
10 21
20 45
30 15
40 10

One of the results recorded was an anomaly.

Identify which result was anomalous and explain your choice.

3c
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1 mark

Describe the trend shown by the data in the table in part (b)

3d
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1 mark

The student evaluated his method and decided that counting bubbles is an inaccurate way of measuring the rate of photosynthesis for two reasons.

  1. It does not take into account the fact that there may be bubbles of different sizes
  2. Tiny bubbles may be hard to see and so may not be counted at all

Suggest a more accurate method which could be used to collect information about the amount of oxygen gas produced as the aquatic plant photosynthesizes.

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4a
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3 marks

Separate: Biology Only

Separate: Higher Tier Only

Sketch a graph to illustrate the relationship between light intensity and the rate of photosynthesis.

4b
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5 marks

Separate: Biology Only

Higher Tier Only

(i)
Using the inverse square law, explain why the rate of photosynthesis decreases as the distance from the light source increases.

(2)

(ii)
Design an experimental setup to demonstrate the inverse square law in a photosynthesis investigation. Include specific equipment and procedures.

(3)

4c
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1 mark

Which factor does not directly limit the rate of photosynthesis?

  A Light intensity
  B Carbon dioxide concentration
  C Temperature
  D Water availability
4d
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3 marks

You have a pondweed (a photosynthesising plant) submerged in sodium hydrogen carbonate solution. Describe how you would use it to investigate the effect of carbon dioxide concentration on the rate of photosynthesis.

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5a
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2 marks

Photosynthesis is an __________ reaction.

Fill in the missing term then define it.

5b
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4 marks
(i)
Calculate the total number of oxygen molecules produced when 12 molecules of carbon dioxide undergo photosynthesis.

(1)

(ii)
Fill in the blanks for the balanced equation to justify your answer.

(3)

12CO2 + 12H2O → …C6H12O6 + …O2

5c
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1 mark

What is the primary function of glucose produced during photosynthesis?

  A Source of energy in respiration
  B Structural component of cell walls
  C Storage as starch
  D Synthesis of lipids for energy in seeds
5d
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3 marks

Complete Table 1 below by matching the correct limiting factor with its effect on the rate of photosynthesis. 

Table 1

Limiting factor Effect on photosynthesis rate
Temperature  
Light intensity  
Carbon dioxide concentration  

A Directly proportional until another factor becomes limiting.
B Accelerates the reaction until another factor limits it.
C Increases kinetic energy of particles and enzyme activity.

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1a
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5 marks

Figure 1 shows a food chain. 

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Figure 1
(i)
Give the name of the trophic level of the small plants. 
(1)
(ii)
The arrows in a food chain represent the flow of energy from one trophic level to the next. 

Describe how the plants gain the energy that they can then pass onto the aphids. 
(2)
(iii)
The energy from this process is converted into biomass in the plant.
The small plants contain 17.5 g per mof biomass and they transfer 20% of this to the aphids.

Calculate the biomass of the aphids in g per m2.
(2)

1b
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4 marks

Figure 2 show the rate of photosynthesis in a field containing small plants, during a 12 hour period. 

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Figure 2

(i)
Describe the trend shown in Figure 2 from 6 am to 6pm. 

(2)

(ii)
Suggest two factors that may affect the rate of photosynthesis during this 12 hour period. 

(2)

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2a
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6 marks

Higher Tier only

A scientist investigated the effect of light intensity on the rate of photosynthesis of the aquatic Cabomba plant. 

A lamp was used as a source of light. The lamp was placed at different distances (d) from the Cabomba plant, and the number of bubbles produced in 60 seconds was used to calculate the rate of photosynthesis. 

The light intensity was then calculated using the inverse square law .

Figure 1 shows the scientist's results. 

distance (d) of lamp from Cabomba (cm) light intensity (arbitrary units) bubbles produced in 60 seconds
5 0.0400 79
10 0.0100 21
15 0.0044 12
20   7
25 0.0016 5
30 0.0011 4

Figure 1

(i)
Calculate the light intensity when the lamp is 20 cm from the Cabomba plant.

(2)

(ii)
Use the information from Figure 1 to describe the effect of light intensity on the rate of photosynthesis. 

(2)

(iii)
The scientist counted the number of bubbles produced by the Cabomba plant.

Explain how you could improve the method to measure the volume of gas released more accurately. 

(2)

2b
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2 marks

The student repeats the experiment, changing the lightbulb to allow for a higher light intensity to be achieved. The student found that despite the higher light intensity that the rate of reaction did not increase any further.

Explain why the rate of photosynthesis did not increase when the light intensity increased. 

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3a
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3 marks

Figure 1 shows a cross section through a leaf. 

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Figure 1

Describe the process that takes place in a leaf to produce oxygen. 

3b
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3 marks

Using Figure 2, state the location where most oxygen is produced, and describe the method by which oxygen leaves the leaf. 

3c
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3 marks

Some of the oxygen does not leave the leaf and instead is used for another reaction within the leaf. 

(i)
State the name of this reaction. 

(1)

(ii)
At what time of day is more oxygen taken into the leaf than removed?

Explain your answer. 

(2)

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4a
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4 marks

The concentration of carbon dioxide in two areas of a rainforest was measured. Each measurement was taken three times. The results are shown in Figure 1.

Area A was full of large trees and area B was an area with a small number of small trees, as it was just recovering from some recent deforestation. 

before-saving

Figure 1

(i)

Calculate the mean carbon dioxide concentration in area B

(2)

(ii)

Explain why all the carbon dioxide concentration readings for area B are higher than for area A

(2)

4b
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2 marks

The experiment was repeated in a high altitude area.

Suggest how the results would vary in the high altitude area compared to the data in Figure 2, and explain how this would affect the biomass of the trees in this area. 

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5a
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4 marks

A student placed some balls made of algae into each of four small glass bottles containing hydrogencarbonate indicator. 

Three of the bottles were sealed with a lid and placed at different distances from a light source. 

The fourth bottle was wrapped in opaque foil in order for the algae to be kept in the dark.

Hydrogencarbonate indicator changes colour due to the amount of carbon dioxide in the solution. 

The investigation was left for 50 minutes and the colour of hydrogencarbonate solution was recorded. 

Figure 1 shows this investigation.

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Figure 1

(i)
State two variables the student will need to control in this investigation. 

(2)

(ii)
State the purpose of the bottle wrapped in foil in this investigation.

(2)

5b
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3 marks

At the start of the investigation the hydrogencarbonate indicator was red in colour.

Figure 2 shows the colour of the hydrogencarbonate indicator in the four bottles at the end of the investigation. 

distance from lamp (cm) colour pH
10 purple 9.2
20 magenta 8.8
30 red 8.5
kept in foil red 8.4

Figure 2

Explain why the hydrogencarbonate indicator in the bottle nearest to the light source turned purple. 

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6
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3 marks

Figure 1 shows the effect of temperature on the rate of photosynthesis. 

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Figure 1

Describe and explain the effect of temperature on the rate of photosynthesis

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7a
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3 marks

Figure 1 shows a cross section through a leaf.fig-12-1bio-2f-june18-qp-gcse-bio

Figure 1

(i)
What is the name of the part labelled R in Figure 1?
(1)
  A cell wall
  B cytoplasm
  C stomata
  D waxy cuticle

(ii)

Figure 2 shows the mass of glucose produced in each layer of a leaf per hour.fig-13-1bio-2f-june18-qp-gcse-bio
Figure 2


Describe the difference in the mass of glucose produced per hour in the palisade mesophyll and the mass of glucose produced in the spongy mesophyll shown in Figure 2.

(2)

7b
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2 marks

Figure 3 shows how light intensity changed during one day.fig-14-1bio-2f-june18-qp-gcse-bio

Figure 3

Use the information in Figure 3 to explain why oxygen moved out of the leaf between 9 am and midday.

7c
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3 marks
(i)

Glucose is produced in a leaf.

Glucose is a

(1)

  A vitamin
  B protein
  C lipid 
  D carbohydrate

(ii)

Describe a test for glucose.
(2)
7d
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1 mark

Figure 4 shows an enzyme and three substrates found in plant cells.fig-15-1bio-2f-june18-qp-gcse-bio

Figure 4

The enzyme will only break down one of these substrates.

State the name of this enzyme.

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8a4 marks

Higher Tier Only

Figure 1 shows the effect of light intensity and temperature on the rate of photosynthesis.

fig-16-1bio-2h-june18-qp-gcse-bio

Figure 1

(i)
Describe the effect of light intensity on the rate of photosynthesis.

(2)

(ii)
Explain the effect of temperature on the rate of photosynthesis.

(2)

8b1 mark

Higher Tier Only

A student measured the rate of photosynthesis using algal balls in a laboratory.

The tube of algal balls was kept at a temperature of 25 °C and was moved to different distances from a light source.

The results of this investigation showed that the rate of photosynthesis is

  A directly proportional to the distance from a light source
  B inversely proportional to light intensity
  C directly proportional to temperature
  D inversely proportional to the distance from a light source
8c6 marks

Explain how substances are moved through a plant by transpiration and translocation.

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9a
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2 marks

Figure 1 shows an area of woodland with some small plants growing in the ground between the trees.

woodland-leaf-litter

Colin Smith, CC BY-SA 2.0 <https://creativecommons.org/licenses/by-sa/2.0>, via Wikimedia Commons

Figure 1

The leaves of the small plants are green.

Describe how these plants make glucose.

9b
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1 mark

Name a group of organisms that break down the dead leaves and release mineral ions into the soil.

9c
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2 marks

The mineral ions are absorbed from the soil into the roots of plants.

Describe how these mineral ions are transported from the roots to the leaves of the plants.

9d
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3 marks

A scientist recorded the mean light intensity and the mean number of small plants per m2 for six 25m2 areas of the woodland.

Figure 2 shows the results.

area of woodland mean light intensity
in lux
mean number of
small plants per m2
A 1500 2.7
B 1300 1.6
C 1000 1.1
D 800 0.6
E 550 0.3
F 350 0.1

Figure 2

(i)

Explain the effect of light intensity on the number of small plants per m2

(2)

(ii)

State one variable the scientist should have controlled to make sure the light intensity measurements could be compared.

(1)

9e
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3 marks

The scientist selects an area near the edge of the woodland where many stinging nettles are growing.

This area is partly shaded by the trees.

Describe how the scientist should use a belt transect to investigate how light intensity affects the growth of stinging nettles.

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1a
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2 marks

An investigation was carried out to test the effect of temperature on the rate of photosynthesis of pondweed.

The volume of oxygen produced per hour was measured. The results can be seen in Table 1.

Table 1

temperature / °C rate of photosynthesis / cm3 hour-1
test 1 test 2 test 3 mean
20 18.4 19.3 19.6 19.1
25 31.6 35.1 31.9 32.8
30 42.9 44.2 44.9 44.0
35 40.6 39.8 42.0 40.8
40 23.1 20.5 22.4 22.0
45 1.9 14.2 2.2 X

Calculate the mean rate of photosynthesis per hour when the temperature was 45 °C.

1b
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5 marks

Plot the results from Table 1 onto an appropriate graph using the graph paper in Fig. 1.

graph-paper-1

Fig. 1

1c
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4 marks

Describe and explain the results shown in the graph.

1d
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4 marks

A farmer growing strawberries in his greenhouse used the results in Table 1 to decide on the best temperature for his greenhouse. He decided to heat the greenhouse to 30 °C.

When the farmer measured the rate of photosynthesis of his plants, he found the rate to be 11.5% slower than the data suggested.

Calculate the rate of photosynthesis that the farmer actually achieved at 30 °C.

1e
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2 marks

Suggest why the farmer may not have achieved a rate of photosynthesis as high as the data in Fig. 1 predicted.

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2a
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4 marks

Figure. 1 shows the effect of several different factors on the growth of strawberry plants in a greenhouse.

limiting-factors-and-photosynthesis-2

Figure. 1

Describe and explain how temperature and light intensity affected the rate of photosynthesis up until 20°C.

2b
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2 marks

Identify the limiting factor(s) between point X and Y in Fig. 1.

2c
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2 marks

Calculate the percentage difference in the rate of photosynthesis at 25°C in the plants grown at low and high light intensity. Show your working and give your answer to one decimal place.

2d
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2 marks

Higher Tier Only

Explain why light intensity is no longer limiting the rate of photosynthesis at temperatures higher than 30°C.

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3a
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2 marks

A student carried out an investigation into the effect of soil magnesium on plant growth. Magnesium is a key vitamin required in the synthesis of chlorophyll.

In this investigation, the student set up two different environments as follows:

  1. One plant was grown in sterile soil, provided with distilled water and placed in bright light.
  2. One plant was grown in sterile soil, provided with magnesium dissolved in distilled water and placed in bright light.

Predict what the student will have observed after two weeks of growing in these conditions.

3b
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4 marks

Explain your prediction from part (a).

3c
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3 marks

When selecting the plants for the investigation in part (a), the student made sure that they picked plants with the same number of leaves.

Explain why this was necessary.

3d
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2 marks

One method that can be used to show that photosynthesis has occurred is to test the leaves with iodine.

Explain how this shows that photosynthesis has occurred.

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4a
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2 marks

Figure 1 shows the changes in carbon dioxide uptake and release over a 24 hour period in a tomato plant.

carbon-dioxide-over-24hrs-1

Figure 1

Explain what the graph shows at 7:30 and 19:30.

4b
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3 marks

Calculate the percentage decrease in carbon dioxide uptake between 15:00 and 18:00 in Figure 1.

4c
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4 marks

Higher Tier Only

With reference to the amount of carbon dioxide released and taken in, Figure 1 can be used to indicate how the rate of photosynthesis changes throughout the 24 hour period, assuming that the rate of respiration is constant during this time.

Explain how interactions between temperature and light intensity contribute towards the changes in photosynthesis represented in Figure 1.

4d
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2 marks

Over 24 hours, the tomato plant takes in more carbon dioxide than it releases.

Explain the value of this to the tomato plant.

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