Forming Urine (Edexcel GCSE Biology)

The kidneys

• The kidneys are located in the back of the abdomen and have two important functions in the body:
  • They regulate the water content of the blood (vital for maintaining blood pressure)
  • They excrete the toxic waste products of metabolism (such as urea) and substances in excess of requirements (such as salts)

Waste substances removed by the kidney

Structure of the kidneys

• There are three regions of the kidney
  • Cortex - the outermost region
  • Medulla - the inner section of the kidney
  • Renal pelvis - the tube linking the kidney to the ureter

• Each kidney contains around a million tiny structures called nephrons, also known as kidney tubules or renal tubules
• Nephrons start in the cortex of the kidney, loop down into the medulla and back up to the cortex
• The contents of the nephrons drain into the renal pelvis and the urine collects there before it flows into the ureter to be carried to the bladder for storage

The structure and location of the kidney

The nephron

• The nephron is made up of a kidney tubule which has several sections:
  • Bowman's capsule
  • Proximal convoluted tubule
  • Loop of Henlé
  • Distal convoluted tubule
  • Collecting duct

• Surrounding the tubule is a network of capillaries with a knotted section which sits inside the Bowman's capsule

Structure of a nephron

• In the kidney, blood is filtered (ultrafiltration) before the key substances are reabsorbed back into the blood again (selective reabsorption)

Ultrafiltration

• Arterioles branch off the renal artery and lead to each nephron, where they form a knot of capillaries (the glomerulus) sitting inside the cup-shaped Bowman’s capsule
• The capillaries get narrower as they get further into the glomerulus which increases the pressure on the blood moving through them (which is already at high pressure because it is coming directly from the renal artery which is connected to the aorta)
• This eventually causes the smaller molecules being carried in the blood to be forced out of the capillaries and into the Bowman’s capsule, where they form what is known as the filtrate
• This process is known as ultrafiltration
• The substances forced out of the capillaries are glucose, water, urea, salts
• Some of these are useful and will be reabsorbed back into the blood further down the nephron

Diagram showing the process of ultrafiltration

Reabsorption of glucose

• After the glomerular filtrate enters the Bowman’s Capsule, glucose is the first substance to be reabsorbed at the proximal (first) convoluted tubule
• This takes place by active transport
• The nephron is adapted for this by having many mitochondria to provide energy for the active transport of glucose molecules
• Reabsorption of glucose cannot take place anywhere else in the nephron as the gates that facilitate the active transport of glucose are only found in the proximal convoluted tubule
• In a person with a normal blood glucose level, there are enough gates present to remove all of the glucose from the filtrate back into the blood
• People with diabetes cannot control their blood glucose levels and they are often very high, meaning that not all of the glucose filtered out can be reabsorbed into the blood in the proximal convoluted tubule
• As there is nowhere else for the glucose to be reabsorbed, it continues in the filtrate and ends up in the urine
• This is why one of the first tests a doctor may do to check if someone is diabetic is to test their urine for the presence of glucose

Reabsorption of water & salts

• As the filtrate drips through the Loop of Henle necessary salts are reabsorbed back into the blood by diffusion
• As salts are reabsorbed back into the blood, water follows by osmosis
• Water is also reabsorbed from the collecting duct in different amounts depending on how much water the body needs at that time

Diagram showing reabsorption in the nephron

Components of Filtrate Table