Cells: Membrane Structure & Transport (DP IB Biology: HL)

Phospholipids are described as amphipathic molecules. Explain what this means.

Liposomes can be used in a range of applications such as the delivery of mRNA vaccines to specific areas of the body.

Sketch a small section of the structure of a liposome indicating its exterior, interior and the hydrophobic and hydrophilic regions.

Describe the properties that allow some molecules to diffuse rapidly across the cell membrane.

The diagram below shows a typical epithelial cell that lines the airways of the lungs. The viscosity of mucus is controlled by the CFTR chloride ion channel. When there is too little water in the mucus the CFTR channel opens allowing Cl^- ions to move into the mucus. When active, the CFTR channel also inhibits the sodium channel and limits movement of Na⁺ ions into the cell. The resulting high concentration of salt in the mucus draws water out of the cell via osmosis which makes the mucus less viscous.

Cystic fibrosis is a genetic disorder characterised by the build-up of thick sticky mucus that causes severe damage to the respiratory and digestive systems. People with class I cystic fibrosis have inherited two faulty versions of the gene for CFTR which results in an absence of the CFTR channel.

Cubes of potato were placed in a solution containing sodium ions. The concentration of oxygen in air bubbled through the solution was changed and the rates of respiration and uptake of sodium ions were measured. The results are shown in the table below.

Describe the relationship between oxygen concentration, rate of respiration and rate of uptake of sodium ions.

Suggest reasons for the relationship between oxygen concentration, rate of respiration and rate of uptake of sodium ions described in part (a).

Give two similarities between the processes by which inorganic ions and water molecules enter cells.

Distinguish between passive and active movement of molecules across the cell membrane.

Some scientists investigated the uptake of magnesium ions in rice plants. They divided the plants into two groups and placed their roots in solutions containing radioactive magnesium ions.

Group Y: plants had a substance that inhibited respiration added to the solution

Group Z: plants did not have the respiratory inhibitor added to the solution

The scientists calculated the total mass of magnesium ions absorbed by the plants every 5 minutes. Their results are shown in the graph below:

Calculate the ratio of the mean rate of uptake of magnesium ions in the first 20 minutes to the mean rate of uptake of magnesium ions in the second 20 minutes for group Z plants.

Using the graph in part (a), calculate the rate of uptake of magnesium ions for group Y plants after 40 minutes. Give suitable units.

Consider the graph in part (a). Explain the results of the investigation.

State two differences between the processes of facilitated diffusion and active transport.

A biologist investigated how surface area affects osmosis in potato cubes.

The results are shown in the graph

Explain why the potato tissue changed in mass.

The biologist recorded the masses of the cubes at intervals. Before weighing the cubes at each interval, the biologist blotted dry the outside of each cube. Explain why.

During the first 20 minutes, the combined loss in mass of the eight small cubes is greater than in the single large cube (as shown in the graph in part a). Calculate the rate of loss in mass per cm² per minute for the single large cube and the eight small cubes during the first 20 minutes. Give your answers in grams per cm² per minute and show your working.

One mark is available for clarity of communication throughout this question.

The drawing below shows an electron micrograph of a neuron. Similar images helped support the Davson and Danielli model of membrane structure.

 Outline the evidence that led to the Davson and Danielli model being rejected.

Draw an annotated diagram of the cell membrane based on the fluid mosaic model including all the relevant structures.

The concentration of sodium ions in red blood cells is lower than the concentration in blood plasma.

Explain how this difference in sodium concentration is maintained.

During pregnancy, the fetus is dependent on essential proteins being absorbed from the mother’s blood in the placenta.

Explain how these proteins would be transported into the fetus.

Describe how the structure of the membrane allows for the transport of proteins from the mother to the fetus.

Discuss the evidence used to falsify the Davson-Danielli model of membrane structure.

Identify the part of the fluid mosaic structure of the plasma membrane represented by the molecular diagram below.

Scientists have found that wheat crops adapted to grow in winter have increased unsaturated phospholipid content.

Suggest why the presence of unsaturated phospholipids would be advantageous.

Pieces of phospholipid bilayer were analysed from two different mammalian cell surface membranes. Sample X contained phospholipid molecules at a density of 4.2 x 10⁶ molecules µm^-2, whereas sample Y contained phospholipid molecules at a density of 5.5 x 10⁶ molecules µm^-2. One sample was from an exocrine pancreatic cell and the other was from a skin cell.

Identify, with reasons, which cell type corresponds to samples X and Y.  

Researchers have discovered that an individual phospholipid molecule can exchange places with its neighbouring phospholipid molecule in a monolayer as frequently as 10⁷ times per second. By contrast, phospholipid molecules almost never exchange places with each other from one monolayer to the other within a bilayer, referred to as a ‘flip-flop’ exchange. The ‘flip-flop’ takes place around once a month for a typical phospholipid molecule.

Suggest why there is this difference in molecular behaviour.

Outline why scientists use detergents to study the structure of membranes. 

Diffusion can be studied using visking tubing. Students set up an investigation in which equal volumes of each of the following solutions were placed into separate visking tubings:

0.7 mol dm^-3 sodium chloride

0.7 mol dm^-3 glucose

The visking tubings, each of the same size, were placed in distilled water and maintained at a constant temperature of 23°C. The volume and mass of the bags were measured at 5 minute intervals for 160 minutes.

The data recorded is shown below.

Calculate the rates of increase in mass and in volume for the visking tubing containing glucose solution during the first 30 minutes.

Compare and contrast the rates of change in mass for the two visking tubings.

Explain why the volume of both visking tubings increases over time.

A biologist investigated the osmolarity of different parts of turnip tubers of different ages (as shown in the diagram below). The osmolarity was estimated using discs of turnip tissue and sucrose solutions of different concentrations.
The diagram below shows:

The appearance of the tubers

The places where the tissues were removed

The results are shown in the graph below

Estimate the osmolarity of the near new shoot sample of the old tuber.

Explain why the biologist used percentage change in mass rather than the change in actual mass.

After analysing the data the biologist came to the following conclusions:

1. The tissue in the old tuber close to the new shoots has the highest osmolarity
2. In the old tuber close to new shoots, starch reserves were being converted to sugar
3. In the old tuber central region, starch was being converted to sugar
4. In the newly formed tuber, all the sugar had been converted to starch.

Evaluate the conclusions made by the biologist based on the evidence collected.

Suggest two possible sources of error that the biologist may have encountered when collecting the data in this investigation.

One mark is available for clarity of communication throughout this question.

Explain the consequences to impulse transmission if mammalian neurons were to stop performing facilitated diffusion of potassium ions.

Outline the effects of putting human heart tissue into a hypotonic solution.

Draw a labelled diagram to show the structure of the fluid mosaic structure containing four different named proteins.