Activation Energy: Skills (DP IB Biology)

Activation Energy

• Metabolic pathways are controlled by enzymes in a biochemical cascade of reactions

  • Virtually every metabolic reaction within living organisms is catalysed by an enzyme

  • Enzymes are therefore essential for life to exist

• Enzymes are biological catalysts

  • ‘Biological’ because they function in living systems

  • ‘Catalysts’ because they speed up the rate of chemical reactions without being used up or undergoing permanent change

The Enzyme-Substrate Complex

• The starting point of a metabolic pathway is a substrate which is converted to an end product

• The enzyme works by binding to the substrate at a special site on the enzyme called the active site

  • The active site of an enzyme has a specific shape to fit a specific substrate

• Substrates collide with the enzyme's active site and this must happen at the correct orientation and speed in order for a reaction to occur

• An enzyme-substrate complex is formed, temporarily, when the substrate binds to the active site

  • The substrate is said to be in a transitional state at this moment

• The product is formed and enzyme is released to take part in another reaction

• The reaction can be shortened to a simple equation

Process of enzyme-catalysed reactions diagram

The simple equation can show how an enzyme reaction proceeds

Formation of enzyme-substrate complex diagram

The formation of the enzyme-substrate complex where the substrate is said to be in a transitional state, before forming the product(s)

Enzymes and the lowering of activation energy

• All chemical reactions, including metabolic pathways, are associated with energy changes

• Energy may either be released or absorbed during a reaction

  • If energy is released to the surroundings it is an exergonic reaction

  • If energy is absorbed from the surroundings it is an endergonic reaction

• For a reaction to proceed there must be enough activation energy

• Activation energy is the amount of energy needed by the substrate to become unstable enough for a reaction to occur and for new products to be formed

• Enzymes speed up chemical reactions because they reduce the stability of bonds in the substrate

• Enzymes lower the activation energy needed to catalyse a reaction

  • The energy released is unchanged but the activation energy required is lowered

  • The rate of reaction is therefore quicker

The effect of enzyme action on activation energy diagram

The graph shows how an enzyme lowers the activation energy required for a reaction