Mitosis: Skills (DP IB Biology)
Revision Note
Identifying Phases of Mitosis
Cells undergoing different stages of the cell cycle can be identified using photomicrographs taken from microscope slides
Cells undergoing certain stages of the cell cycle have distinctive appearances
Interphase
As cells spend the majority of the cell cycle in this stage then most cells will be in this stage
The chromatin is visible (however chromosomes are not) so the nuclei have a dark appearance
Prophase
Chromosomes are visible
The nuclear envelope is breaking down
Metaphase
Chromosomes are lined up along the middle of the cell
Anaphase
Chromosomes are moving away from the middle of the cell, towards opposite poles
As they are pulled from the centromere through the cytoplasm, the chromosomes tend to have a characteristic 'V' shape
Telophase
Chromosomes have arrived at opposite poles of the cell
Chromosomes begin to uncoil (are no longer condensed)
The nuclear envelope is reforming
Cytokinesis
Animal cells: a cleavage furrow forms and separates the daughter cells
Plant cells: a cell plate forms at the site of the metaphase plate and expands towards the cell wall of the parent cell, separating the daughter cells
Identification of phases
Micrograph showing a cell undergoing prophase (P)
Micrograph showing cells undergoing metaphase (M) and anaphase (A)
Micrograph showing cells undergoing metaphase (M) and anaphase (A)
Micrograph showing a cell undergoing anaphase (A)
Examiner Tips and Tricks
It is important to be able to recognise each mitotic stage from electron micrographs and to be able to explain why that cell is in the stage you have selected. It can be difficult to tell prophase and telophase apart in some photomicrographs. In prophase, there is only one group of chromosomes in the cell while in telophase there are two groups, one at each pole.
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