Cell Types & Structures: Skills
- It is important to be able to recognise various organelles from light and electron microscope images
- When interpreting micrographs to identify and deduce the function of the cell it is important to:
- Identify whether it is a prokaryotic or eukaryotic cell - look to see if a nucleus is present or not
- Identify which type of eukaryotic cell it is (plant or animal) by looking for a cell wall or vacuole
- Identify the organelles present in the cells and consider their function
- You should be confident in identifying the following structures and organelles:
- Nucleoid region in a prokaryotic cell
- Prokaryotic cell wall
- Nucleus
- Mitochondria
- Chloroplast
- Sap vacuole
- Golgi apparatus
- Rough and smooth endoplasmic reticulum
- Chromosomes
- Ribosomes
- Plant cell wall
- Plasma membrane
- Microvilli
- Some identifiable features of key organelles are:
- Chloroplast
- Has distinctive stacks of thylakoids
- Double membrane
- Has a roughly oval shape
- Larger than mitochondria
- Indicates the cell is a plant cell
- Nucleus
- Has a nuclear membrane and a dark nucleolus within
- It has a roughly spherical shape
- Vacuole
- Occupies a large space within a cell
- Often shows up as a very light shade (white) within an electron micrograph
- Indicates the cell is a plant cell
- Cell wall
- Located around the perimeter of the cell
- Mitochondria
- Roughly oval-shaped
- Double membrane
- Sometimes observed with visible cristae (foldings of the inner membrane)
- Chloroplast
An interpretation of an electron micrograph of a cell
Electron micrograph of cell 1
- To identify this cell consider the following
- The cell has a nucleus - therefore it is a eukaryotic cell
- This cell does not have a cell wall or central vacuole - therefore it is an animal cell
- The cell has a large u-shape nucleus - so it can manipulate itself through small pores
- There are a large number of lysosomes in the cell - so it can digest substances found within the cell
- There are a large number of mitochondria - this means it has sufficient energy for its many metabolic reactions
- The deduction, therefore, is that this cell needs a lot of energy to break down substances that enter the cell and that it can move where it wants. This cell is a macrophage
An interpretation of an electron micrograph of a cell
Electron micrograph of cell 2
- To identify this cell consider the following
- The cell has a nucleus - therefore it is a eukaryotic cell
- This cell does not have a cell wall or central vacuole - therefore it is an animal cell
- There are a large number of mitochondria - so it requires significant energy for many metabolic reactions
- The cell has microvilli packed closely together (brush border) - so it needs to increase the surface area and prevent any substance from crossing into the cell
- The deduction, therefore, is that this cell needs a lot of energy to control what enters or exits this cell and that the cell requires many of the substance to be absorbed. This cell is a ciliated epithelium of the small intestine
Worked example
The image below shows a cell as viewed through an electron microscope.
Identify three organelles and the type of cell.
Answer:
- Identifiable organelles are:
- Nucleus and nucleolus
- Mitochondria
- Chloroplast
- Vacuole
- Cell wall
- This is a plant cell