Non-competitive Inhibition
Enzyme inhibitors
- Inhibitors are chemical substances that can bind to an enzyme and reduce its activity
- Inhibitors can be formed from within the cell or can be introduced from the external environment
- An enzyme's activity can be reduced or stopped, temporarily, by an inhibitor
- There are two types of inhibitors: competitive and non-competitive
Allosteric sites and non-competitive inhibitors
- Non-competitive inhibitors bind to the enzyme at an alternative site which is not the active site
- These sites are called allosteric sites and they are usually located quite far from the active site
- Only specific substances (called effectors) can bind to an allosteric site
- Binding to the allosteric site is reversible
- Binding to the allosteric site causes interactions within an enzyme which leads to conformational changes
- These conformational changes will alter the shape of the active site
- This therefore prevents the substrate from binding to the active site
- This will apply for as long as the effector is bound to the allosteric site
Non-competitive inhibition diagram
Non-competitive inhibitors bind to the allosteric site of an enzyme to alter the active site