Syllabus Edition

First teaching 2023

First exams 2025

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Practice Paper 1A (HL IB Biology)

Practice Paper Questions

1
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Which row of the table lists the four common metabolites in decreasing order of solubility in water?

  • oxygen → sodium chloride → cholesterol → hydrophobic amino acid

  • sodium chloride → oxygen→ hydrophobic amino acid  → cholesterol

  • hydrophobic amino acid → oxygen → sodium chloride → cholesterol

  • sodium chloride → hydrophobic amino acid → oxygen → cholesterol

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2
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What property of fatty acids made them likely candidates for early cell membranes?

  • Their amphipathic nature

  • Their ability to store genetic information

  • Their catalytic properties

  • Their ability to self-replicate

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3
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The table shows some possible units for measuring alveoli, white blood cells and cell walls.

Which of the rows show the most suitable units for measuring each of these structures?

  Diameter of alveoli Diameter of white blood cells Width of cell walls
A. mm µm µm
B. mm µm nm
C. µm nm µm
D. µm µm nm

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    4
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    Which of the following diagrams show bacteriophage lambda?

    • virus-structure-1

    • virus-structure-2

    • virus-structure-3

    • virus-structure-4

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    5
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    Which of the following presents a difficulty when applying the biological species concept?

    • Sexual reproduction

    • Horizontal gene transfer

    • Gamete incompatibility

    • Linnaean classification

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    6
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    Which of the following cannot be concluded from the cladogram below?

    KQwZdvoK_3

    • Chimps and bonobos are more closely related to each other than to any other species of primate.

    • Chimps, bonobos, humans, and gorillas form a clade.

    • Lemurs diverged from the rest of the primates around 56 million years ago.

    • Chimps, bonobos, humans, and orangutans form a clade.

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    7
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    A team of palaeontologists discovered the remains of an insect-like organism trapped in amber, which was found in rock layers believed to be about 180 million years old. As they dug deeper into the older layers of rock, they made several further discoveries. Their findings are shown in the diagram below.q7_10-3_gene_pools_speciation_ib_biology_hl_mcq

    What conclusion can be drawn from the information given?

    • The changes observed in these populations occurred gradually from 180 million years ago until 500 million years ago.

    • The original population changed dramatically over time which led to several different species forming

    • There were several large speciation events that occurred within a short period of time within these populations

    • Evolutionary change occurred gradually, with many small changes accumulating over a long period of time

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    8
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    Which of the following is an important factor in mixed dipterocarp ecosystem loss in Southeast Asia?

    • Introduction of non-native species.

    • Establishment of nature reserves.

    • Clear-cutting of trees to provide agricultural land.

    • Changes in atmospheric oxygen levels.

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    9
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    A study was conducted to investigate the effect of saturated fat intake on the risk of developing coronary heart disease (CHD) in women.

    • The study involved 20 women, aged between 35 and 50, divided into two groups (A and B) of 10.
    • Group A was given a diet rich in saturated fats, while group B followed a diet low in saturated fats.
    • The levels of low-density lipoprotein (LDL) in their blood was monitored over a six month period. High LDL levels has been linked to an increase in the risk of developing CHD.

    At the end of the study the levels of LDL in group A was much higher than that of group B. The scientists concluded that saturated fats increases the risk of developing CHD.

    Which evaluation of their conclusion would be most valid?

    • The conclusion of the scientist may not be valid as they would have to repeat the investigation over a longer period of time, using animal test subjects

    • The conclusion of the scientists may not be valid as they should have monitored a larger number of women between the ages of 20 and 35

    • The conclusion of the scientists may not be valid as they should have taken into consideration the medical history of the women

    • The conclusion of the scientists may not be valid as they should have repeated their investigation with women over the age of 75

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    10
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    The following diagram shows a dipeptide with various parts of the molecule numbered (1 to 4).

    e-1

    Which row of the table below correctly identifies each labelled part of this dipeptide?

      1 2 3 4
    A. Carboxyl group Peptide bond R-group Amino group
    B. R-group Amino group Peptide bond Carboxyl group
    C. Amino group Carboxyl group Peptide bond R-group
    D. Amino group Peptide bond R-group Carboxyl group

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      11
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      What is the difference between simple diffusion and facilitated diffusion?

        Simple diffusion Facilitated diffusion
      A. Diffusion rate inversely proportional to concentration gradient Diffusion rate proportional to concentration gradient
      B. Never involves a membrane Always involves a membrane
      C. Occurs across any part of a membrane Occurs via channels in the membrane
      D. A passive process that does not require energy from ATP An active process that requires energy from ATP

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        12
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        The cube below has sides that measure 2 cm in length. e-q4

        Which of the following represents the correct surface area : volume ratio of this cube?

        • 1:1

        • 6:1

        • 3:1

        • 2:1

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        13
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        The diagram shows a trace recorded from a spirometer.

        5ktomJeq_4Which label represents the tidal volume?

         

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          14
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          Certain environmental factors can affect the rate of transpiration in plants. The effect of one environmental factor on transpiration rate is shown in the graph below.q9_9-1_transport_in_the_xylem_of_plants_ib_biology_hl_mcq

          Identify the environmental factor that is having this effect on transpiration rate.

          • Temperature

          • Humidity

          • Air movement

          • Light intensity

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          15
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          Girdling, or ring-barking, involves the removal of a strip of bark around the entire circumference of a tree trunk. This removes the outer part of the vascular bundles which results in the death of the entire tree over time.

          What could be a possible explanation for this?

          • Xylem is removed from the vascular bundles, so water and mineral salts cannot be transported to the leaves

          • Phloem is removed from the vascular bundles, so sugars cannot be transported to the roots

          • Xylem is removed from the vascular bundles, so sugars cannot be transported to the roots

          • Phloem is removed from the vascular bundles, so water and mineral salts cannot be transported to the leaves

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          16
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          The aquatic crustacean the water flea (Daphnia magna, pictured below) has a heart that pumps blood-like liquid called hemolymph around its body cavity, once for every complete circuit. 
          wOdjGLLP_h-3

          Which statement about Daphnia magna's circulatory system is correct?

          • single closed

          • single open

          • double closed

          • double open

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          17
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          The diagram below shows the sarcomere of a muscle fibre.

          q4_11-2_movement_ib_biology_hl_mcq

          Which of the following statements correctly describes the events observed during a muscle contraction?

          • The length of the sarcomere stays the same, the dark band gets longer and the light bands stay the same

          • The length of the sarcomere gets shorter, the dark band gets shorter and the distance between the two Z lines gets shorter

          • The length of the sarcomere stays the same, the dark band gets longer and the distance between the two Z lines gets shorter

          • The length of the sarcomere gets shorter, the dark band stays the same and the light bands get shorter

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          18
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          Which row correctly describes an example of an adaptation for survival in the given biome?

            Biome Adaptation
          A. Tropical forest Thick waxy cuticles on leaves
          B. Temperate forest Nocturnal behaviour
          C. Taiga Wide buttress roots
          D. Hot desert The ability to extract water from food

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            19
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            The Ghost Orchid (Epipogium aphyllum) is a rare plant species which lacks leaves or chlorophyll. The plant has formed a symbiotic relationship with a fungus in order to obtain the nutrition it requires. The growth of the fungus is slowed as a result of the relationship.

            Which of the following rows correctly describes E.aphyllum?

              Heterotroph Autotroph Parasite Saprotroph
            A X X
            B X X
            C X
            D X X

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              20
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              A bacterial enzyme from the species Caldicoprobacter algeriensis can be immobilised onto polymethacrylate beads for the conversion of glucose into fructose in the food industry, as shown in the diagram below.q8-2-4-enzymes-medium-ib-hl-biology

              What is the name of the enzyme that is immobilised on the polymethacrylate beads?

              • Glucose isomerase.

              • Fructose convertase.

              • Fructose isomerase.

              • Glucose convertase.

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              21
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              The activity of two types of protease enzymes was measured in two different species of fish; species X and species Y. Protease enzymes are responsible for breaking down proteins into amino acids. The results of the research are shown in the graph.

              The unit U denotes the enzyme required for the production of 1 μmol of product per minute. The bars represent the standard deviation.YI4IEjOY_h-1

              Which statement about protease enzymes is correct?

              • The activity of the stomach protease of species Y is 2.5 times greater than that of species X.

              • The activity of the intestinal protease of species X is significantly higher than the stomach protease of species X.

              • The activity of the stomach protease of species X is 3.5 times less than that of species Y.

              • The activity of the intestinal proteases of both species X and Y are significantly higher than the stomach proteases.

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              22
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              The diagram below shows a simplified Krebs cycle.

              How many times does decarboxylation occur during the Krebs cycle?q5_8-2_cell_respiration_ib_biology_hl_mcq

              • Once

              • Twice

              • Three times

              • Four times

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              23
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              A student designed the following experimental set-up to measure the effect of light intensity on the rate of photosynthesis. The experiment was repeated three times.

              biology-2-8-q7-ib-hl

              Distance from lamp (cm) Number of bubbles per minute
              Replicate 1 Replicate 2 Replicate 3
              10 99 81 64
              20 63 43 45
              30 31 18 13
              40 18 7 8
              50 15 6 4


              The results were inconsistent, how could the experimental set-up be improved?

              • Measuring the change in biomass of the plant.

              • Using cooled, boiled water to remove dissolved oxygen.

              • Replacing the water with sodium hydrogen carbonate solution.

              • Using a red light source.

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              24
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              Which process is not part of the light-independent reaction of photosynthesis?

              • Glycerate-3-phosphate is converted to triose phosphate

              • Rubisco is regenerated from triose phosphate

              • ATP is dephosphorylated to ADP

              • Carboxylation of RuBP

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              25
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              Which features are characteristic of transmembrane receptor proteins?

                External ligand binding site Hydrophilic amino acids Hydrophobic phospholipid tails Non-polar Intracellular domains
              A
              B
              C
              D

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                26
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                The diagram below shows a sodium-potassium pump within the axon membrane of a neurone.

                nDcY0Z0__4

                What is the role of active transport in the transmission of nerve impulses by neurones?

                • Establishes the resting potential needed for impulse transmission by pumping sodium ions out of the axon and potassium ions into the axon.

                • Depolarisation of the axon by moving sodium ions across the membrane into neurone.

                • Establishes the resting potential needed for impulse transmission by moving sodium ions into the axon and potassium ions out of the axon.

                • Initiates the action potential needed for the transmission of an impulse by pumping calcium ions into the axon.

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                27
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                The diagram below shows the left to right transmission of an action potential in a myelinated axon by saltatory conduction.qeahNoHs_h-2

                Which of the following rows correctly identifies I - IV?

                  I II III IV
                A. Node at resting potential Node at action potential Node at refractory period Node becoming depolarised
                B. Node at refractory period Node at action potential Node becoming depolarised Node at resting potential
                C. Node at action potential Node at refractory period Node becoming depolarised Node at resting potential
                D. Node at refractory period Node becoming depolarised Node at action potential Node at resting potential

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                  28
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                  A pot plant falls over and after a few days shows the following growth pattern.q3_9-3_growth_in_plants_ib_biology_hl_mcq

                  Which of the following is the most accurate explanation for the growth shown at each of the labels on the diagram?

                  • This part of the stem demonstrates negative gravitropism in order to receive the maximum amount of sunlight for photosynthesis

                  • This part of the stem shows positive phototropism as it will bend towards the light

                  • The lateral roots demonstrates negative gravitropism as they are not growing towards gravity

                  • The main root shows positive gravitropism as it is bending towards gravity

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                  29
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                  Some bacteria develop resistance to antibiotics by forming biofilms, which are large colonies of bacteria clumped together.

                  Which statement best explains how forming a biofilm might give a bacterial species resistance to antibiotics?

                  • Antibiotic molecules are physically unable to reach all bacterial cells in a biofilm

                  • Biofilms secrete chemicals which break down the antibiotic

                  • Biofilms remain in a host organism for many years

                  • Bacterial cells in a biofilm go into a suspended state but can become infectious again at a later stage

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                  30
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                  What can be concluded about levels of atmospheric carbon dioxide from the graph shown?wx0jijoF_1

                  A

                  Carbon dioxide levels fluctuate yearly due to seasonal changes in photosynthesis

                  Average carbon dioxide levels are increasing

                  Average yearly carbon dioxide level increases vary from year to year

                  B

                  Carbon dioxide levels fluctuate yearly

                  Average carbon dioxide levels increased between 1960-2010

                  Average yearly carbon dioxide level increases vary from year to year

                  C

                  Carbon dioxide levels fluctuate yearly

                  Average carbon dioxide levels are increasing

                  Average yearly carbon dioxide level increases vary from year to year

                  D

                  Carbon dioxide levels fluctuate yearly due to seasonal changes in photosynthesis

                  Average carbon dioxide levels increased between 1960-2010

                  Average yearly carbon dioxide level increases are linear

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                    31
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                    A DNA profile was created for two genes in three individuals (A, B, and C).7-1_q2h

                    Which of the following statements, relating to the images provided, are correct?

                    I.

                    The relationship between DNA fragment length and distance moved through the gel is linear.

                    II.

                    Fragment length is determined by the number of repeated DNA sequences in a sample.

                    III.

                    There is a percentage increase of 50 % between the distance moved by fragment Y and that moved by fragment X.

                    IV.
                    Individual A is homozygous for both of the genes tested.

                    • I, II, and III only.

                    • I, II, III, and IV.

                    • II and IV only.

                    • III and IV only.

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                    32
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                    A piece of DNA was analysed to find the number of nucleotide bases in each of the polynucleotide strands.  Some of the results are shown below.

                      Number of nucleotide bases
                      A T G C
                    Strand 1   30 22  
                    Strand 2   30 38  

                    What is the maximum number of amino acids that could be coded for by this DNA?

                    • 30

                    • 35

                    • 40

                    • 50

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                    33
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                    The nucleotide base sequence and the associated sequence of four amino acids can be seen below:

                    AGG   ACA   CCT   GGA

                    Serine   Tyrosine   Glycine   Proline

                    The table shows the mRNA codons and their associated amino acids.

                    h-1

                    A single mutation occurred in the original base sequence of the DNA which resulted in only one amino acid from the sequence being produced.

                    Which of the following would represent the correct nucleotide base sequence which was found in the DNA after the mutation occurred?

                    • AGT   ACA   CCT   GGA

                    • AGG   ACT   CCT   GGA

                    • AGG   ACC   CCT   GGA

                    • AGG   ACA   UGA   GGA

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                    34
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                    In humans, during periods of hypoxia (low oxygen in tissues) the transcription factor HIF (Hypoxia-Inducible Factor) binds to the Hypoxia Response Element (HRE) sequence and increases transcription of the EPO gene. This stimulates the production of red blood cells. The diagram shows the EPO gene and regulatory elements.                                                                                   q2_7-2_transcription_gene_expression_ib_biology_hl_mcq

                    Which kind of regulatory elements are HIF and HRE?

                      HIF HRE
                    A

                    Activator protein

                    Enhancer sequence

                    B

                    Repressor protein

                    Silencer sequence

                    C

                    Enhancer sequence

                    Activator protein

                    D

                    General transcription factor

                    Enhancer sequence

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                      35
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                      An experiment to calculate the water potential of potato cells was performed using the following protocol:

                      1. Dice potato into 1cm3 cubes.
                      2. Add potato cubes to 6 test tubes, each containing solution with a different sucrose concentration.
                      3. Wait 12 hours.
                      4. Weigh the cubes from each test tube and plot a graph. 

                      The mean mass of the potato cubes was 2.50 g before being added to the solution, and the graph shows the masses of the potato cubes after the experiment. 1-3-h-q4-paper-1

                      Which concentration of sucrose matches the water potential of the potato cells?

                      • 0.00 mol dm-3

                      • 0.12 mol dm-3

                      • 0.31 mol dm-3

                      • 0.66 mol dm-3

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                      36
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                      Two linked genes are denoted by the dominant alleles G and H. Their recessive counterparts are g and h respectively.

                      Which way of writing the allele combinations on their chromosomes best displays the linkage between the two genes?

                      • q1a_10-2_inheritance_ib_biology_hl_mcq

                      • q1b_10-2_inheritance_ib_biology_hl_mcq

                      • q1c_10-2_inheritance_ib_biology_hl_mcq

                      • q1d_10-2_inheritance_ib_biology_hl_mcq

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                      371 mark

                      Nephrogenic syndrome of inappropriate antidiuresis (NSIAD) is a rare condition that can be caused by a gain-of-function mutation in the gene that codes for ADH receptor proteins. This means that sufferers of the condition will produce more ADH receptors or will produce ADH receptors that are more sensitive than normal.

                      Which of the following will not be true for sufferers of NSIAD?

                      • The kidney cells will respond to ADH even when ADH levels are low.

                      • The body cells will swell up.

                      • The kidney cells will have more water transport proteins in their cell surface membranes than normal.

                      • Large amounts of dilute urine will be produced.

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                      38
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                      The three graphs below show how the allele frequencies of two alleles in three different populations changed over several generations. 10-3_q3h

                      Which of the graphs show changes in allele frequency that suggest that evolution is taking place?

                      • II. only

                      • II. and III. only

                      • III. only

                      • I., II. and III. 

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                      39
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                      Which of the statements about ecological succession is correct?

                      • Succession can only be triggered by changes in abiotic conditions.

                      • During primary succession the arrival of new species can alter the abiotic conditions present in a habitat.

                      • Succession always results in a stable climax community.

                      • A climax community will always be more diverse than the ecosystems that occur during the process of succession.

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                      40
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                      The graph below shows the differences between the radiation reaching the earth from the sun and the radiation re-emitted by the earth.g~NDX832_3

                      How does this radiation cause the greenhouse effect?

                      • Greenhouse gases absorb mainly low intensity radiation.

                      • Greenhouse gases absorb mainly shortwave radiation.

                      • Greenhouse gases absorb mainly UV radiation.

                      • Greenhouse gases absorb mainly longwave radiation.

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