Sago pondweed is an underwater plant that grows in many regions of the world.
Fig. 1.1 shows a transmission electron micrograph of a sago pondweed cell.
Fig. 1.1
(i) Identify the cellular components shown at A and B.
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(ii) The real size of the line between C and D on Fig. 1.1 is 1.4 × 10−6 m.
Calculate the magnification that was used to produce the image in Fig. 1.1.
Give your answer to 2 significant figures.
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(iii) Fig. 1.2 shows a student’s drawing of another sago pondweed cell, which was observed under a light microscope. The student used a sharp pencil but did not label the drawing.
Fig. 1.2
Describe two other ways in which the drawing could be improved.
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(iv) The student stained a sago pondweed sample to improve the contrast between cellular components when viewed under a microscope.
The student used the following procedure to stain the sample:
Use forceps to place the sample on a glass slide.
Use a pipette to place two drops of the stain in the centre of the sample.
Carefully lower a cover slip onto the sample, ensuring that the cover slip is parallel with the slide as it is lowered.
Describe two improvements the student should make to their staining procedure.
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