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Rate: Substrate Concentration (CIE AS Biology)

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Rate: Substrate Concentration

  • The greater the substrate concentration, the higher the rate of reaction:
    • As the number of substrate molecules increase, the likelihood of enzyme-substrate complex formation increases
    • If the enzyme concentration remains fixed but the amount of substrate is increased, past a certain point all available active sites eventually become saturated and any further increase in substrate concentration will not increase the reaction rate
    • When the active sites of the enzymes are all full, any substrate molecules that are added have nowhere to bind in order to form an enzyme-substrate complex and so the reaction rate will not increase any further until active sites become free again
  • For this reason, in the graph below there is a linear increase in reaction rate as substrate is added, which then plateaus when all active sites become occupied

Substrate Concentration Diagram

The effect of substrate concentration on an enzyme-catalysed reaction, downloadable AS & A Level Biology revision notes

The effect of substrate concentration on the rate of an enzyme-catalysed reaction

Examiner Tip

If substrate concentration is continually increased but enzyme concentration is kept constant, there eventually comes a point where every enzyme active site is working continuously. At this point, the substrate molecules are effectively ‘queuing up’ for an active site to become available. At this stage, the enzyme is working at its maximum possible rate, known as Vmax (V stands for velocity).

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Cara Head

Author: Cara Head

Expertise: Biology

Cara graduated from the University of Exeter in 2005 with a degree in Biological Sciences. She has fifteen years of experience teaching the Sciences at KS3 to KS5, and Psychology at A-Level. Cara has taught in a range of secondary schools across the South West of England before joining the team at SME. Cara is passionate about Biology and creating resources that bring the subject alive and deepen students' understanding