Enzymes (Cambridge (CIE) AS Biology): Revision Note

Exam code: 9700

Cara Head

Written by: Cara Head

Reviewed by: Alistair Marjot

Updated on

Enzymes as proteins

  • Enzymes are biological catalysts

    • ‘Biological’ because they function in living systems

    • ‘Catalysts’ because they speed up the rate of chemical reactions without being used up or changed

  • Enzymes are also globular proteins

  • Critical to the enzyme's function is the active site where the substrate binds

  • Metabolic pathways are controlled by enzymes in a biochemical cascade of reactions

    • Virtually every metabolic reaction within living organisms is catalysed by an enzyme – enzymes are therefore essential for life to exist

  • Enzymes can be intracellular or extracellular referring to whether they are active inside or outside the cell respectively

    • Intracellular enzymes are produced and function inside the cell

    • Extracellular enzymes are secreted by cells and catalyse reactions outside cells (e.g. digestive enzymes in the gut)

 

Intracellular

Extracellular

Example

Catalase

Amylase

Function

Hydrogen peroxide is produced as a by-product of many cellular reactions

It is harmful to cells

Catalase converts hydrogen peroxide into water and oxygen, preventing cell damage

Digestion of food is carried out by extracellular enzymes

This is because macromolecules of food are too large to enter cells

Amylase is an an example of a digestive enzyme

It digests carbohydrates into simple sugars

Examiner Tips and Tricks

Don't forget that enzymes are proteins and so anything that could denature a protein, rendering it non-operational (extremes of heat, temperature, pH etc.) would also denature an enzyme.

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Cara Head

Author: Cara Head

Expertise: Biology & Psychology Content Creator

Cara graduated from the University of Exeter in 2005 with a degree in Biological Sciences. She has fifteen years of experience teaching the Sciences at KS3 to KS5, and Psychology at A-Level. Cara has taught in a range of secondary schools across the South West of England before joining the team at SME. Cara is passionate about Biology and creating resources that bring the subject alive and deepen students' understanding

Alistair Marjot

Reviewer: Alistair Marjot

Expertise: Environmental Systems and Societies & Biology Content Creator

Alistair graduated from Oxford University with a degree in Biological Sciences. He has taught GCSE/IGCSE Biology, as well as Biology and Environmental Systems & Societies for the International Baccalaureate Diploma Programme. While teaching in Oxford, Alistair completed his MA Education as Head of Department for Environmental Systems & Societies. Alistair has continued to pursue his interests in ecology and environmental science, recently gaining an MSc in Wildlife Biology & Conservation with Edinburgh Napier University.