Syllabus Edition

First teaching 2023

First exams 2025

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Enzymes (CIE AS Biology)

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Cara Head

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Enzymes as Proteins

  • Enzymes are biological catalysts
    • ‘Biological’ because they function in living systems
    • ‘Catalysts’ because they speed up the rate of chemical reactions without being used up or changed
  • Enzymes are also globular proteins
  • Critical to the enzyme's function is the active site where the substrate binds
  • Metabolic pathways are controlled by enzymes in a biochemical cascade of reactions
    • Virtually every metabolic reaction within living organisms is catalysed by an enzyme – enzymes are therefore essential for life to exist
  • Enzymes can be intracellular or extracellular referring to whether they are active inside or outside the cell respectively
    • Intracellular enzymes are produced and function inside the cell
    • Extracellular enzymes are secreted by cells and catalyse reactions outside cells (e.g. digestive enzymes in the gut)

Enzymes Table

  Intracellular Extracellular
Example Catalase Amylase
Function
  • Hydrogen peroxide is produced as a by-product of many cellular reactions
  • It is harmful to cells
  • Catalase converts hydrogen peroxide into water and oxygen, preventing cell damage
  • Digestion of food is carried out by extracellular enzymes
  • This is because macromolecules of food are too large to enter cells
  • Amylase is an an example of a digestive enzyme
  • It digests carbohydrates into simple sugars

Examiner Tip

Don't forget that enzymes are proteins and so anything that could denature a protein, rendering it non-operational (extremes of heat, temperature, pH etc.) would also denature an enzyme.

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Cara Head

Author: Cara Head

Expertise: Biology

Cara graduated from the University of Exeter in 2005 with a degree in Biological Sciences. She has fifteen years of experience teaching the Sciences at KS3 to KS5, and Psychology at A-Level. Cara has taught in a range of secondary schools across the South West of England before joining the team at SME. Cara is passionate about Biology and creating resources that bring the subject alive and deepen students' understanding