DNA, RNA & Protein Synthesis (College Board AP® Biology)

Exam Questions

13 mins7 questions
1a2 marks
qp4-2016-frq-ap-biology

The figure represents the process of expression of gene X in a eukaryotic cell.

The primary transcript in the figure is 15 kilobases (kb) long, but the mature mRNA is 7 kb in length. Describe the modification that most likely resulted in the 8 kb difference in length of the mature mRNA molecule. Identify in your response the location in the cell where the change occurs.

1b2 marks

Predict the length of the mature gene X mRNA if the full-length gene is introduced and expressed in prokaryotic cells. Justify your prediction.

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2a1 mark

Researchers are studying the use of RNA vaccines to protect individuals against certain diseases. To develop the vaccines, particular cells are first removed from an individual. Then mRNAs coding for specific proteins from a pathogen are introduced into the cells. The altered cells are injected back into the individual, where the cells make the proteins encoded by the introduced mRNAs. The individual then produces an immune response to the proteins that will help to protect the individual from developing a disease if exposed to the pathogen in the future.

When introduced into cells, the mRNAs used for vaccines must be stable so that they are not degraded before the encoded proteins are produced. Researchers developed several modified caps that they hypothesized might make the introduced mRNAs more stable than mRNAs with the normal GTP cap. To test the effect of the modified caps, the researchers produced mRNAs that differed only in their cap structure (no cap, the normal cap, or modified caps I, II, or Ill). They introduced the same amount of each mRNA to different groups of cells and measured the amount of time required for half of the mRNAs to degrade (mRNA half-life) and the total amount of protein translated from the mRNAs (Table 1).

TABLE 1. EFFECT OF mRNA CAP STRUCTURE ON mRNA HALF-LIFE AND PROTEIN TRANSLATED FROM THE INTRODUCED mRNA

5' Cap Structure

mRNA Half-Life  ±2 SE subscript top enclose x end subscript  (hours after introduction into cells)

Total Amount of Protein
Translated from
mRNA ±2 SE subscript top enclose x end subscript (relative to
amount in normal cap)

No cap

1.41 ± 0.02

0.011 ± 0.000

Normal GTP cap

16.10 ± 1.83

1.000 ± 0.007

Modified cap I

15.50 ± 1.57

4.777 ± 0.042

Modified cap II

27.00 ± 2.85

13.094 ± 0.307

Modified cap III

18.09 ± 0.81

6.570 ± 0.075

Based on the data, identify which cap structure is most likely to protect the end of the mRNAs from degradation.

2b1 mark

Based on the data for the mRNAs with modified caps, describe the relationship between the mRNA half-life and the total amount of protein produced.

2c1 mark

After examining the data on mRNA half-lives and the amount of protein produced, the researchers hypothesized that each mRNA molecule with modified cap I was translated more frequently than was each mRNA molecule with the normal GTP cap. Evaluate their hypothesis by comparing the data in Table 1.

2d1 mark

The introduction of mRNAs into cells allows the cells to produce foreign proteins that they might not normally produce. Explain why the production of a foreign protein may be more likely from the introduction of mRNA than DNA into cells.

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