Evidence for Evolution (College Board AP® Biology): Study Guide

Ruth Brindle

Written by: Ruth Brindle

Reviewed by: Cara Head

Updated on

Evidence for evolution

  • The theory of evolution by natural selection is now widely accepted by the scientific community due to its being supported by a great deal of evidence

Data that provides evidence for evolution

  • Geological evidence examines fossil distribution and rock strata to trace organism changes over time

  • Geographical evidence links organism distribution to shared ancestry

    • E.g. finches in the Galápagos Islands and honeycreepers in the Hawaiian Islands demonstrate adaptive radiation

Diagram of birds evolving from a common extinct ancestor, originating in Hawaii; arrows point to various evolved species in different colours.
The Hawaiian honeycreepers show continuous variation across their geographical range, suggesting that they diverged gradually from a common ancestor
  • Physical evidence includes fossils and homologous structures

    • E.g. transitional fossils like Archaeopteryx, a bird-like dinosaur fossil which provides a bridge between dinosaurs and current-day birds

  • Biochemical evidence uses DNA and protein sequence comparisons

    • E.g. cytochrome c sequence similarities across species indicate common ancestry

  • Mathematical models calculate divergence times and genetic similarity

    • E.g. molecular clocks measure mutation rates in genes to estimate divergence

      • The greater the number of differences there are between nucleotide sequences, the longer ago the common ancestor of both species existed

Evidence of changes in organisms over time

Fossil dating methods

  • The age of fossils can be determined using radioactive isotopes to date the fossils or by studying the age of the layers where the fossils are found

    • E.g. naturally occurring carbon-14 in an organism decays at a known rate, based on the half-life of the carbon 14 isotope

    • Scientists measure the level of carbon-14 in fossilized organisms to estimate how long ago that organism died

  • Geographical data considers the physical location of species and fossil distribution which allows scientists to establish where common ancestors link different species together

  • A combination of fossil dating and geographical data can be used to build timelines of evolution

Morphological homologies

  • Homologous structures are similar structures which have different functionalities despite common ancestry

    • E.g. the pentadactyl limb shows structural similarity as well as adaptation

  • Vestigial structures, e.g. pelvic bones in whales and wings in flightless bird, suggest evolutionary leftovers from common ancestors

Illustration comparing limb bones of a human, whale, bird, frog, and alligator, highlighting similarities in skeletal structure.
The bone structure of the pentadactyl limb of a human, whale, bird, frog, and alligator; they all have the same basic layout despite having evolved for different functions

Molecular and cellular evidence

  • Similarities in DNA nucleotide sequences, e.g. Hox genes, and protein amino acid sequences, e.g.hemoglobin, show evolutionary relationships between different species

  • Higher sequence similarity indicates a closer relationship between species and a more recent divergence from a common ancestor

Diagram of primate evolution showing common ancestors and speciation, with branches to humans, chimpanzees, bonobos, gorillas, and orangutans.
The results of DNA and protein sequencing enable evolutionary trees to be built that show the evolutionary relationships between species
  • Conserved processes

    • Similarities in cellular processes e.g. DNA replication, cellular respiration, and protein synthesis highlight shared ancestry

  • Structural evidence

    • Common uses of structural cell components suggest evolutionary links

      • Ribosomes in all cells perform protein synthesis

      • Shared genetic codes and membrane structures (e.g. phospholipid bilayers) indicate a common origin

Evidence for common ancestry

Eukaryotic ancestry

  • Structural evidence indicates common ancestry of all eukaryotes:

    • Membrane-bound organelles (e.g., nucleus, mitochondria, chloroplasts) indicate a shared origin in eukaryotic organisms

    • Linear chromosomes in eukaryotes contrast with circular DNA in prokaryotes

    • The presence of introns in eukaryotic genes supports a common eukaryotic lineage

      • E.g., endosymbiotic theory explains how mitochondria and chloroplasts originated from prokaryotes

Continuing evolution

  • Life continues to evolve indefinitely driven by changing environments

  • There are many observations in the modern day which demonstrate this continual evolution, e.g.:

    • genomic changes

      • Mutations and genetic drift drive divergence over time where greater differences between organisms indicate a more distant common ancestor

    • fossil records

      • Evidence from fossils shows continuous change in traits over time

    • resistance development

      • Mutations are acquired which provide a survival advantage, demonstrating natural selection in action.

      • Evolution of antibiotic-resistant bacteria like MRSA demonstrates natural selection in action

      • Plants and animals evolve resistance to pesticides, e.g. DDT, or herbicides

      • Cancer cells show an increasing resistance to chemotherapy drugs

    • emerging pathogens

      • Seasonal flu variations evolve every year through mutations which alter antigens and cause current vaccinations to become ineffective

      • Diseases like COVID-19 show rapid evolution of pathogens due to environmental pressures

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Ruth Brindle

Author: Ruth Brindle

Expertise: Biology

Ruth graduated from Sheffield University with a degree in Biology and went on to teach Science in London whilst also completing an MA in innovation in Education. With 10 years of teaching experience across the 3 key science disciplines, Ruth decided to set up a tutoring business to support students in her local area. Ruth has worked with several exam boards and loves to use her experience to produce educational materials which make the mark schemes accessible to all students.

Cara Head

Author: Cara Head

Expertise: Biology Content Creator

Cara graduated from the University of Exeter in 2005 with a degree in Biological Sciences. She has fifteen years of experience teaching the Sciences at KS3 to KS5, and Psychology at A-Level. Cara has taught in a range of secondary schools across the South West of England before joining the team at SME. Cara is passionate about Biology and creating resources that bring the subject alive and deepen students' understanding