Chromatography is a technique used to separate the components of a mixture.
A sample of a tripeptide was hydrolysed and then placed on a thin layer chromatography (TLC) plate. Samples of possible amino acids present were also placed on the TLC plate for reference.
A simplified diagram of the developed TLC plate is shown.
A – Lysine | B – Serine | C – Histidine | D – Tyrosine |
E – Isoleucine | F – Methionine | G – Proline | H – Hydrolysed tripeptide |
(1)
☐ | A | iodine | |
☐ | B | methyl orange | |
☐ | C | ninhydrin | |
☐ | D | phenolphthalein |
Gas chromatography can be used both to separate the components in a mixture and to determine the amount of each present.
(3)
Did this page help you?