Students investigated the effect of light on the growth of garden cress seedlings.
A total of 120 seedlings were divided into 2 groups of 60.
Group A was grown in darkness for 2 days.
Group B was grown for 1 day in darkness and then for 1 day in white light using the set‐up shown in Fig. 3.1.
Fig. 3.1
The results of the students’ experiment are shown in Tables 3.1 and 3.2.
Group | Mean length (mm) | Mean mass (μg) | ||
stem | root | stem | root | |
A | 13 | 18 | 102 | 60 |
B | 25 | 23 | 160 | 120 |
Table 3.1
Direction of growth in Group B | Number of seedlings | |
stem | root | |
Away from light | 2 | 29 |
Neither away from nor towards light | 3 | 20 |
Towards light | 55 | 11 |
Table 3.2
(i) Describe and explain the results shown in Tables 3.1 and 3.2.
[6]
(ii) The students wanted to test whether there was a significant difference between the stem lengths of the seedlings in Group A and the seedlings in Group B.
State the name of the most appropriate statistical test for the students to use.
[1]
(iii) Justify your choice of statistical test given in part (ii).
[1]
(iv) Table 3.2 records the direction of growth as:
away from light
neither away from nor towards light
towards light.
The students used the chi‐squared test to determine whether the direction of root growth was significantly different from their expectations.
Their null hypothesis was:
There is no difference between the expected direction of root growth and the observed direction of root growth.
The calculated chi‐squared value was 8.10.
The students compared their chi‐squared value of 8.10 to the values in Table 3.3.
Degrees of freedom | Probability (p) | ||
0.10 | 0.05 | 0.01 | |
1 | 2.71 | 3.84 | 6.64 |
2 | 4.60 | 5.99 | 9.21 |
3 | 6.25 | 7.82 | 11.34 |
4 | 7.78 | 9.49 | 13.28 |
5 | 9.24 | 11.07 | 15.09 |
Table 3.3
What can the students conclude about their results based on a chi‐squared value of 8.10?
[3]
The solution in which the seedlings were grown contained various dissolved ions.
The table below lists two of the functions of these ions in the seedlings.
Complete the table below with the formula of each ion.
Function | Formula of ion |
Supplies elements that form part of the structure of amino acids and nucleotides |
|
Forms part of the structure of DNA and phospholipids |
|
The students also investigated the effect of plant hormone concentration on root growth.
(i) State the name of a plant hormone that would be expected to affect root growth.
[1]
(ii) In the investigation, the students controlled light, temperature and mineral concentration.
State one other factor that the students should have controlled in this investigation.
[1]
The growth of plant roots is thought to be controlled by specialised cells called statocytes.
One hypothesis for how a statocyte controls root growth involves small organelles called amyloplasts and is shown in Fig. 3.2.
Fig. 3.2
What can you conclude from the information in Fig. 3.2 about how a statocyte controls root growth?
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