Eukaryotic Cells (OCR A Level Biology): Revision Note

Eukaryotic Cell Structure

Cell surface membrane

The structure of the cell surface membrane – although the structure looks static the phospholipids and proteins forming the bilayer are constantly in motion

• All cells are surrounded by a cell surface membrane which controls the exchange of materials between the internal cell environment and the external environment

  • The membrane is described as being ‘partially permeable’

• The cell membrane is formed from a phospholipid bilayer of phospholipids spanning a diameter of around 10 nm

Cell wall

The cell wall is freely permeable to most substances (unlike the plasma membrane)

• Found in plant cells but not in animal cells

• Cell walls are formed outside of the cell membrane and offer structural support to cell

• Structural support is provided by the polysaccharide cellulose in plants, and peptidoglycan in most bacterial cells

• Narrow threads of cytoplasm (surrounded by a cell membrane) called plasmodesmata connect the cytoplasm of neighbouring plant cells

Nucleus

The nucleus of a cell contains chromatin (a complex of DNA and histone proteins) which is the genetic material of the cell

• Present in all eukaryotic cells (except red blood cells), the nucleus is relatively large and separated from the cytoplasm by a double membrane (the nuclear envelope) which has many pores

• Nuclear pores are important channels for allowing mRNA and ribosomes to travel out of the nucleus, as well as allowing enzymes (eg. DNA polymerases) and signalling molecules to travel in

• The nucleus contains chromatin (the material from which chromosomes are made)

  • Chromosomes are made of sections of linear DNA tightly wound around proteins called histones

• Usually, at least one or more darkly stained regions can be observed – these regions are individually termed ‘nucleolus’ (plural: nucleoli) and are the sites of ribosome production

Mitochondria

A single mitochondrion is shown – the inner membrane has protein complexes vital for the later stages of aerobic respiration embedded within it

• The site of aerobic respiration within all eukaryotic cells, mitochondria are just visible with a light microscope

• Surrounded by double-membrane with the inner membrane folded to form cristae

• The matrix formed by the cristae contains enzymes needed for aerobic respiration, producing ATP

• Small circular pieces of DNA (mitochondrial DNA) and ribosomes are also found in the matrix (needed for replication)

Chloroplasts

Chloroplasts are found in the green parts of a plant – the green colour a result of the photosynthetic pigment chlorophyll

• Found in plant cells

• Larger than mitochondria, also surrounded by a double-membrane

• Membrane-bound compartments called thylakoids containing chlorophyll stack to form structures called grana

• Grana are joined together by lamellae (thin and flat thylakoid membranes)

• Chloroplasts are the site of photosynthesis:

  • The light-dependent stage takes place in the thylakoids

  • The light-independent stage (Calvin Cycle) takes place in the stroma

• Also contain small circular pieces of DNA and ribosomes used to synthesise proteins needed in chloroplast replication and photosynthesis

Ribosomes

Ribosomes are formed in the nucleolus and are composed of almost equal amounts of RNA and protein

• Found in all cells

• Found freely in the cytoplasm of all cells or as part of the rough endoplasmic reticulum in eukaryotic cells

• Each ribosome is a complex of ribosomal RNA (rRNA) and proteins

• 80S ribosomes (composed of 60S and 40S subunits) are found in eukaryotic cells

• 70S ribosomes (composed of 50S and 30S subunits) in prokaryotes, mitochondria and chloroplasts

• Site of translation (protein synthesis)

Endoplasmic reticulum

The RER and ER are visible under the electron microscope - the presence or absence of ribosomes helps to distinguish between them

Rough Endoplasmic Reticulum (RER)

• Found in plant and animal cells

• Surface covered in ribosomes

• Formed from continuous folds of membrane continuous with the nuclear envelope

• Processes proteins made by the ribosomes

Smooth Endoplasmic Reticulum (ER)

• Found in plant and animal cells

• Does not have ribosomes on the surface, its function is distinct to the RER

• Involved in the production, processing and storage of lipids, carbohydrates and steroids

Golgi apparatus (golgi complex)

The structure of the Golgi apparatus

• Found in plant and animal cells

• Flattened sacs of membrane similar to the smooth endoplasmic reticulum

• Modifies proteins and lipids before packaging them into Golgi vesicles

  • The vesicles then transport the proteins and lipids to their required destination

  • Proteins that go through the Golgi apparatus are usually exported (e.g. hormones such as insulin), put into lysosomes (such as hydrolytic enzymes) or delivered to membrane-bound organelles

Large permanent vacuoles

The structure of the vacuole

• A sac in plant cells surrounded by the tonoplast, selectively permeable membrane

• Vacuoles in animal cells are not permanent and small

Vesicles

The structure of the vesicle

• Found in plant and animal cells

• A membrane-bound sac for transport and storage

Lysosomes

The structure of the lysosome

• Specialist forms of vesicles which contain hydrolytic enzymes (enzymes that break biological molecules down)

• Break down waste materials such as worn-out organelles

• Used extensively by cells of the immune system and in apoptosis (programmed cell death)

Centrioles

The structure of the centriole

• Hollow fibres made of microtubules

• Two centrioles at right angles to each other form a centrosome, which organises the spindle fibres during cell division

• Not found in flowering plants and fungi

Microtubules

The structure of the microtubule

• Found in all eukaryotic cells

• Makes up the cytoskeleton of the cell about 25 nm in diameter

• Made of α and β tubulin combined to form dimers, the dimers are then joined into protofilaments

  • Thirteen protofilaments in a cylinder make a microtubule

• The cytoskeleton is used to provide support and movement of the cell

Microvilli

The structure of the microvilli

• Found in specialised animal cells

• Cell membrane projections

• Used to increase the surface area of the cell surface membrane in order to increase the rate of exchange of substances

Cilia

The structure of the cilia

• Hair-like projections made from microtubules

• Allows the movement of substances over the cell surface

Flagella

The structure of the flagella

• Found in specialised cells

• Similar in structure to cilia, made of longer microtubules

• Contract to provide cell movement for example in sperm cells