Syllabus Edition

First teaching 2023

First exams 2025

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Energy (CIE A Level Biology)

Exam Questions

2 hours12 questions
1a
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3 marks

Fig. 1 shows the percentage daily usage of the energy ingested by a typical adult male.

This man has a moderately active lifestyle and shows typical eating/drinking behaviour.

The person's typical total energy output is 10 500 kJ day-1

BLcbZQo1_cie-ial-12-12-q1ae---sq

Fig. 1

Calculate the energy, in kJ, that this man expends in maintaining his breathing and heartbeat.

State your answer to the nearest whole number of kilojoules. 

1b
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3 marks

Fig. 1 shows that energy is required for transporting substances across membranes.

(i)

Give one example of a type of membrane transport that requires energy.

[1]

(ii)

One specific example of a membrane transport process that requires energy is the absorption of amino acids in the digestive system.

Give two other specific examples of membrane transport processes in humans that require energy

[2]

1c
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1 mark

Chemical reactions in living organisms can be anabolic or catabolic. Catabolic reactions involve the break down of large molecules into smaller molecules.

Contrast the energy characteristics of anabolic and catabolic reactions in cells, tissues and organs.

1d
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3 marks

Fig. 2 shows a molecule of ATP.

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Fig. 2

Identify the parts of the ATP molecule labelled X, Y and Z in Fig. 2.

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2a
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1 mark

List the molecules ADP, AMP and ATP according to the number of phosphate groups that they possess.

Start with the smallest number of phosphate groups and work upwards.

2b
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1 mark

Aerobic respiration of one molecule of glucose generates roughly 38 molecules of ATP.

Give one advantage of gaining energy for cellular processes in small packets from many ATP molecules, rather than all at once from one molecule of glucose.

2c
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1 mark

Name the enzyme which catalyses the synthesis of ATP.

2d
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2 marks

Describe the process by which ATP can be recycled in the cell.

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3a
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2 marks

Malic acid (C4H6O5) is a substance found naturally in all living organisms and which contributes to the flavours of certain foods such as fruit.

Its respiration by yeast is an important reaction in the process of winemaking.

The balanced chemical equation for the aerobic respiration of malic acid is shown in Fig. 1.

C4H6O5  +  3O2  →  4CO2  +  3H2O

Fig. 1

Calculate the respiratory quotient (RQ) for malic acid. 

3b
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2 marks

The respiratory quotient (RQ) of a food substance known as substance X was measured experimentally as 0.72.

(i)

Give the name of the piece of equipment used to measure RQ.

[1]

(ii)

Suggest the major food group to which substance X belongs.

[1]

3c
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2 marks

The equipment named in part (b) (i) can contain a substance known as soda-lime (sodium hydroxide solution).

State the purpose of soda-lime in this equipment.

3d
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2 marks

Using the equipment from part (b) (i) to demonstrate the measurement of RQ to a class, a teacher was given a choice between an experiment using germinating seeds or small invertebrates such as woodlice.

Both are known to give measurable results. The teacher chose to use the germinating seeds. 

Comment on why the teacher's choice of the germinating seeds was the better option. 

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4a
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6 marks

Fig. 1 represents the process by which ATP is used and regenerated within cells.

cycling-of-atp-and-adp-condensation-hydrolyisis

Fig. 1

(i)

Give the full name of molecule in Fig. 1.

[1]

(ii)

Identify the types of reactions taking place at A and B in Fig. 1.

[2]

(iii)

Identify three processes that make use of the 'energy for cells' indicated in Fig. 1.

[3]

4b
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2 marks

ATP is a useful energy currency for cells because it can be regenerated, as shown in Fig. 1.

Explain one other feature of ATP that makes it especially well suited for its role as energy currency.

4c
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2 marks

There are two types of processes by which ATP can be synthesised within cells.

Identify the two processes by which ATP is synthesised.

4d
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1 mark

Different respiratory substrates have different energy values, meaning that they yield different numbers of ATP molecules.

State one difference between respiratory substrates that influences energy value.

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1a
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2 marks

Producers such as cacti and grasses form the foundation of most desert food webs. Cacti and grasses are consumed by the primary consumers, which in turn are eaten by the secondary consumers.

Fig. 1 shows the flow of energy through a desert ecosystem. The figures in the circles represent the energy transfer in kJ m-2 yr-1.

12-1-fig-1-1

Fig. 1

Calculate the percentage of energy transferred from the primary consumers to the secondary consumers in Fig. 1

Give your answer as a whole number.

1b1 mark

Describe the energy conversion occurring between the sun and the primary consumers in Fig. 1

1c5 marks

All living organisms require energy in order to perform the functions necessary for life.

Outline the need for energy in living organisms; your answer should include suitable examples.

1d3 marks

Photosynthesis and respiration are important processes occurring in living organisms.

Explain how the processes of photosynthesis and respiration assist in the transfer of energy between autotrophs and heterotrophs, with reference to Fig. 1.

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2a1 mark

Fig.1 shows the structure of a molecule of adenosine triphosphate (ATP).

12-1-fig-2-1Fig. 1

Identify part X of the ATP molecule in Fig.1.

2b2 marks

ATP is described as a 'universal energy currency' for cells.

Explain why ATP can be considered to be a 'universal energy currency'.

2c3 marks

Discuss the main benefits of ATP as an energy currency in living organisms.

2d2 marks

ATP is broken down by a group of enzymes known as ATPases.

(i)

State the type of reaction that is catalysed by ATPases.

[1]

(ii)

Identify the products of the reaction mentioned in part (i).

[1]

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3a2 marks

A student set up a respirometer, as illustrated in Fig. 1, to measure the rate of oxygen consumption during respiration in woodlice.

12-1-fig-5-1

Fig. 1

From Fig. 1:

(i)

Identify the control in this experiment.

[1]

(ii)

State the purpose of the potassium hydroxide solution.

[1]

3b3 marks

The level of the coloured liquid was equal on both sides of the capillary u-tube of the manometer at the beginning of the experiment.

Explain the movement of the liquid in the capillary tube, as illustrated in Fig. 1.

3c
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2 marks

The student found that the coloured liquid in the capillary tube in Fig. 1 moved 20 mm over the course of an hour. The diameter of the capillary tube was 2 cm.

The volume of the capillary tube can be calculated by using the formula: πr2h, where h represents the distance moved by the manometer fluid in a minute.

Calculate the volume of oxygen consumed in cm3min-1. Show your working.

3d2 marks

The student repeated the experiment from Fig.1, but removed the potassium hydroxide solution. The volume of gas was calculated again and these values were used to calculate the RQ value of the respiratory substrate of the woodlice.

The RQ value was 0.8.

Suggest what the student can deduce from this RQ value.

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4a
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3 marks

Some hibernating mammals undergo multiple periods of torpor (reduced activity), interspersed with periods of wakefulness, throughout their hibernation.

Research was carried out into the physiological changes occurring in a species of mammal during a period of torpor.

Some of the results are shown in Fig. 1 below.

Graph showing changes in respiratory quotient, body temperature and metabolic rate during a period of torpor

Fig. 1

(i)

Explain the relationship between metabolic rate and body temperature shown in Fig. 1

[2]

(ii)

Suggest the time period during which the mammal enters a state known as 'steady torpor'.

[1]

4b
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3 marks

Describe the changes in respiratory quotient shown in Fig. 1.

4c
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3 marks

Suggest what can be concluded from Fig. 1 about respiratory substrates and torpor.

4d
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2 marks

Suggest the benefit to mammals of brief periods of torpor and arousal, rather than extended periods of unbroken hibernation.

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1a
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1 mark

Fig. 1 shows two processes (A and B) by which ATP can be synthesised.

12-1-fig-3-1
Fig. 1

Identify processes A and B.

1b
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3 marks

Contrast process A and B in Fig. 1 with regards to ATP synthesis.

1c
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3 marks

Only process A in Fig. 1 can occur in anaerobic conditions.

Explain why process B in Fig. 1 cannot occur without the presence of oxygen.

1d
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2 marks

The average human synthesises more than 50 kg of ATP each day. 

Suggest the reasons for synthesising such a large mass of ATP.

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2a
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5 marks

Table 1 shows the amount of energy released per gram of different respiratory substrates.

Table 1

Respiratory substrate Energy value / kJ g-1
Carbohydrate 15.8
Lipid 39.4
Protein 17.0

With reference to Table 1, explain the difference in the energy released by the different respiratory substrates.

2b
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3 marks

Palmitic acid is a saturated fatty acid found in palm oil, cheese and milk. It can be broken down during aerobic respiration, as represented by the following partially-completed equation:

C16H32O2   +   O2 →    CO2   +   H2O

Calculate the respiratory quotient (RQ) of palmitic acid.

Show your working.

2c
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2 marks

The RQ value for glucose is 1.0

Discuss the difference in the RQ values of glucose and palmitic acid.

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3a
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3 marks

Fig. 1 shows a respirometer set up to measure the rate of respiration in germinating mung bean seeds.

It consists of two linked tubes, 1 and 2, held in a temperature-controlled water bath. 

Respirometer Question CIE A Level

Fig. 1

The capillary tubes in the U-tube containing coloured liquid have an internal diameter of 1 mm.

(i)

Explain why the volumes of potassium hydroxide (KOH) are different in tubes 1 and 2. 

[2]

(ii)

Suggest an experimental modification to allow the volumes of potassium hydroxide (KOH) to be the same in tubes 1 and 2. 

[1]

3b
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3 marks

Experimenters used the equipment in Fig. 1 to measure the rate of respiration in 10 g of active mung bean seeds over a 20 minute period.

They calculated a rate of movement of the liquid in the U-tube of 4mm3 hr-1 g-1.

Calculate how far, to the nearest mm, the liquid in the U-tube moved in the 20 minute period of their investigation.

3c
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2 marks

Following the investigation in part (b) the scientists reset the respirometer and ran the experiment again, this time with distilled water in place of KOH in both tubes 1 and 2.

They recorded a slight upward movement of the liquid in the U-tube manometer on the side of tube 2, this time at a rate of 0.72 mm3 hr-1 g-1.

Calculate the respiratory quotient (RQ) of the respiring mung bean seeds in this investigation. 

3d
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2 marks

Suggest, with a reason, what the value of RQ calculated in part (c) indicates about the respiratory substrate of the mung beans at the time of this investigation.

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4a
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6 marks

Explain why carbohydrates, lipids and proteins have different relative energy values as substrates in respiration in aerobic conditions.

4b
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9 marks

Define the term respiratory quotient (RQ) and describe how you would carry out an investigation to determine the RQ of germinating barley seeds.

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