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First exams 2025

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Osmosis in Plant Cells (CIE A Level Biology)

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Osmosis: Plant Cells

  • Osmosis is the net movement of water molecules from a region of higher water potential (dilute solution) to a region of lower water potential (concentrated solution), through a selectively permeable membrane
  • If a plant cell is placed in pure water or a dilute solution, water will enter the plant cell through its selectively permeable cell surface membrane by osmosis, as the pure water or dilute solution has a higher water potential than the plant cell
  • As water enters the vacuole of the plant cell, the volume of the plant cell increases
  • The expanding protoplast (living part of the cell inside the cell wall) pushes against the cell wall and pressure builds up inside the cell – the inelastic cell wall prevents the cell from bursting
  • The pressure created by the cell wall also stops too much water entering and this also helps to prevent the cell from bursting
  • When a plant cell is fully inflated with water and has become rigid and firm, it is described as fully turgid
  • This turgidity is important for plants as the effect of all the cells in a plant being firm is to provide support and strength for the plant – making the plant stand upright with its leaves held out to absorb sunlight
  • If plants do not receive enough water the cells cannot remain rigid and firm (turgid) and the plant wilts

Osmosis of Water into a Plant Cell - Diagram

Osmosis of water into plant cell

Osmosis of water into a plant cell

  • If a plant cell is placed in a solution with a lower water potential than the plant cell (such as a concentrated sucrose solution), water will leave the plant cell through its selectively permeable cell surface membrane by osmosis
  • As water leaves the vacuole of the plant cell, the volume of the plant cell decreases
  • The protoplast gradually shrinks and no longer exerts pressure on the cell wall
  • As the protoplast continues to shrink, it begins to pull away from the cell wall
  • This process is known as plasmolysis – the plant cell is plasmolysed

Plasmolysis of Plant Cells - Diagram

plasmolysis-in-a-plant-cell

Plasmolysis of a plant cell that has been placed in a solution with a lower water potential than the cell itself<

Examiner Tip

Remember – plant cell membranes are composed of a phospholipid bilayer and are selectively permeable (only certain molecules can cross), whereas plant cell walls are made of cellulose and are freely permeable. Thus, in a plasmolysed cell, the external solution will be exerting pressure on the protoplast, that is, there is not an empty space between the cell wall and protoplast. Pure water has a water potential of 0 kPa, so all other solutions will have negative water potentials.

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Phil

Author: Phil

Expertise: Biology

Phil has a BSc in Biochemistry from the University of Birmingham, followed by an MBA from Manchester Business School. He has 15 years of teaching and tutoring experience, teaching Biology in schools before becoming director of a growing tuition agency. He has also examined Biology for one of the leading UK exam boards. Phil has a particular passion for empowering students to overcome their fear of numbers in a scientific context.