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First exams 2025

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Pearson's Linear Correlation (CIE A Level Biology)

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Pearson's Linear Correlation

  • When recording the abundance and distribution of species in an area different trends may be observed
  • Sometimes correlation between two variables can appear in the data
    • Correlation is an association or relationship between variables
    • There is a clear distinction between correlation and causation: a correlation does not necessarily imply a causative relationship
    • Causation occurs when one variable has an influence on, or is influenced by, another

  • There may be a correlation between species; for example, two species always occurring together
  • There may be a correlation between a species and an abiotic factor, for example, a particular plant species and the soil pH
  • The apparent correlation between variables can be analysed using scatter graphs and different statistical tests

Correlation between variables

  • In order to get a broad overview of the correlation between two variables the data points for both variables can be plotted on a scatter graph
  • The correlation coefficient (r) indicates the strength of the relationship between variables
  • Perfect correlation occurs when all of the data points lie on a straight line with a correlation coefficient of 1.0 or -1.0
  • Correlation can be positive or negative
    • Positive correlation: as variable A increases, variable B increases
    • Negative correlation: as variable A increases, variable B decreases

  • If there is no correlation between variables the correlation coefficient will be 0

Types of Correlation Sketch Graphs

Types of correlation graph

Different types of correlation in scatter graphs

  • The correlation coefficient (r) can be calculated to determine whether a linear relationship exists between variables and how strong that relationship is

Pearson linear correlation

  • Pearson's linear correlation is a statistical test that determines whether there is linear correlation between two variables
  • The data must:
    • Be quantitative, e.g. the number of individuals has been counted and a numerical value recorded
    • Show a linear relationship upon visual inspection
    • Show a normal distribution
  •  Method:
      • Step 1:  Create a scatter graph of data gathered and identify if a linear correlation exists
      • Step 2:  State a null hypothesis
      • Step 3:  Use the following equation to work out Pearson’s correlation coefficient r

Pearsons Equation, downloadable AS & A Level Biology revision notes

Where:

  • r = correlation coefficient
  • x =  number of species A
  • y = number of species B
  • n = number of readings
  • Sx = standard deviation of species A
  • Sy = standard deviation of species B
  • x̄= mean number of species A
  • ȳ= mean number of species B
  • If the correlation coefficient r is close to 1.0 or -1.0 then it can be stated that there is a strong linear correlation between the two variables and the null hypothesis can be rejected

Worked example

Some students used quadrats to measure the abundance of different plant species in a garden. They noticed that two particular species seemed to occur alongside each other. They plotted a scatter graph and the data they collected had no major outliers and showed roughly normal distribution.Pearsons worked example graphs

Scatter graph showing the linear correlation between the abundance of species A and B. It shows linear correlation and so is suitable for analysis by Pearson’s correlation coefficient.

Investigate the possible correlation using Pearson’s linear correlation coefficient.

Null hypothesis: There is no correlation between the abundance of species A and species B.

Steps to calculate the correlation coefficient:

Step 1: Calculate xy

Step 2: Calculate  x̅ and y̅ (these are the means of x and y)

Step 3: Calculate nx̅y̅

Step 4: Find ∑xy

Step 5: Calculate standard deviation for each set of data Sx  and Sy

Step 6: Substitute the appropriate numbers into the equation

Pearsons Equation, downloadable AS & A Level Biology revision notes

Quadrat No. of individuals of species A (x) No. of individuals of species B (x) xy
1 10 21 210
2 11 19 209
3 11 22 242
4 6 15 90
5 8 16 128
6 14 24 336
7 10 19 190
8 12 24 288
9 11 21 231
10 10 19 190
Mean x̄ = 10.3 ȳ = 20 ∑xy = 2114
nx̄ȳ 10 × 10.3 × 20 = 2060  
Standard deviation Sx = 2.16 Sy = 3.02  
  • n = 10 as there are 10 quadrat samples
  • The sum of x x y (∑xy) = 2114
  • n x mean of x x mean of y = nx̅y̅ = 2060
  • Sx = 2.16 and Sy = 3.02
  • Substitute values into the equation above:

 r equals fraction numerator 2114 minus 2060 over denominator open parentheses 10 minus 1 open parentheses 2.16 close parentheses open parentheses 3.02 close parentheses close parentheses end fraction equals 0.92

    • As the value of r lies close to 1, the null hypothesis can be rejected
    • There is a strong positive correlation between the abundance of species A and species B

Examiner Tip

You will be provided with the formula for Pearson’s linear correlation in the exam. You need to be able to carry out the calculation to test for correlation, as you could be asked to do this in the exam. You should understand when it is appropriate to use the different statistical tests that crop up in this topic, and the conditions in which each is valid.

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Phil

Author: Phil

Expertise: Biology

Phil has a BSc in Biochemistry from the University of Birmingham, followed by an MBA from Manchester Business School. He has 15 years of teaching and tutoring experience, teaching Biology in schools before becoming director of a growing tuition agency. He has also examined Biology for one of the leading UK exam boards. Phil has a particular passion for empowering students to overcome their fear of numbers in a scientific context.