Benedict’s solution can be used to carry out a semi-quantitative test on a reducing sugarsolution to determine the unknown concentration of reducing sugar present in a Sample. The intensity of any colour change seen relates to the concentration of reducing sugar present in the sample. Using serial dilution, standard solutions with know concentrations of a reducing sugar (such as glucose) are produced.
Each solution is then treated in the same way: the same volume of Benedict’s solution is added to each sample and heat is applied. Any colour change observed for each solution of a known concentration in given time can be attributed to the concentration of reducing sugar present in that solution
The same procedure is carried out on a sample with an unknown concentration of reducing sugar which is then compared to the stock solution colours to estimate the concentration of reducing sugar present
The colour change intensity can be compared between samples by eye or by using a colorimeter. Describe the relationship between colour change intensity and glucose concentration.