Practical Skill: Investigating the Effects of Random Sampling on Allele Frequencies
- Investigations to mimic the effects of random sampling on allele frequencies in a population can be devised using coloured beads, marbles or even sweets
- An example of one such investigation is described below
Apparatus
- 24 beads in one colour and 24 beads in a different colour
- Jar or container
- Paper
- Pencil
- Calculator
Method
- Place 24 red beads and 24 white beads in a container
- Red beads represent a dominant allele (R)
- White beads represent a recessive allele (r)
- Shake the container to ensure the beads are well mixed
- Carry out a random 'mating' by drawing out two beads without looking
- Each pair of beads drawn represents the fusing of a pair of sex cells containing either red or white alleles
- Carry out 24 of these random 'matings' and note down the genotype of each offspring
- Ensure you place the beads back into the container after each draw
- The theoretical offspring genotype ratio (also known as the expected genotype ratio) if thousands of draws are made and there is no genetic drift should be 1:2:1
- RR = 25% (chance of drawing two red beads in a row)
- Rr = 50% (chance of drawing a red bead then a white bead or a white bead then a red bead)
- rr = 25% (chance of drawing two white beads in a row)
- A table of these theoretical results (for 24 random 'matings') is shown below:
Results
- However, the chances of drawing exactly six double red beads (RR), twelve red and white beads (Rr) and six double white beads (rr), as shown in the table above, are very small
- The effects of chance can mean that the offspring genotype ratio is likely to differ from the theoretical 1:2:1 offspring genotype ratio
- This change in the expected offspring genotype ratio that occurs in a small breeding population is known as genetic drift
- The table below shows some example results from 24 random '"matings"
- If these results now represent the alleles in a new population, the ratio of red and white alleles are different from the 24 red alleles and 24 white alleles in the original population:
- Number of red alleles in new population = 5 x 2 (from RR) + 7 (from Rr) = 17
- Number of white alleles in new population = 12 x 2 (from rr) + 7 (from Rr) = 31
- As the white allele frequency has increased to 31 and the red allele frequency has decreased to 17, genetic drift has occurred
- If 24 more random 'matings' are now carried out from the new population (i.e. by placing 17 red beads and 31 white beads in the container), the effect of genetic drift on the allele frequencies in the population can become even greater
- The table below shows some example results from this second round of 24 random 'matings'
- Number of red alleles in new population = 4 x 2 (from RR) + 5 (from Rr) = 13
- Number of white alleles in new population = 15 x 2 (from rr) + 5 (from Rr) = 35
- As the white allele frequency has now further increased to 35 and the red allele frequency has now further decreased to 13, an even greater amount of genetic drift has occurred