Models & Functions of Enzyme Action (AQA A Level Biology)

Revision Note

Test yourself
Lára

Author

Lára

Last updated

Students should be able to: Appreciate the Models & Functions of Enzyme Action

Models of enzyme action

  • Scientists often use models to explain their observations from experiments
  • As technology and research advances within a field new models can be developed and old ones disproven
  • The lock and key model covered at GCSE was originally thought to be an accurate model of enzyme action
    • It suggested that the rigid shape of the active site of the enzyme was a precise fit for the specific shape of the substrate

  • New techniques have allowed scientists to discover that proteins are not rigid structures
    • Experiments showed that multiple regions of an enzyme molecule moved in response to the environment
    • Many of these movements were minimal but some of them were more significant
    • The larger movements occurred when the substrate bound to the enzyme

  • These findings led to the now widely accepted induced fit model
    • Prior to binding, the substrate and active site and not completely complementary in shape
    • When the substrate binds the active site alters shape and moulds around the substrate

  • There is evidence to support the induced fit model:
    • X-ray diffraction techniques allow for 3D pictures of molecules to be formed
    • This technique was used to produce pictures of the enzyme hexokinase before and after it bound to its substrate glucose
    • The images confirmed that the active site of the enzyme changed shape after the substrate bound

_Lock and key hypothesis, downloadable AS & A Level Biology revision notes

The lock-and-key hypothesis

Induced fit hypothesis, downloadable AS & A Level Biology revision notes

The induced-fit hypothesis

Examiner Tip

Enzymes have a wide variety of functions within organisms. They catalyse both intracellular and extracellular reactions which determine the structures and functions of not only cells but the whole organism!

You've read 0 of your 10 free revision notes

Unlock more, it's free!

Join the 100,000+ Students that ❤️ Save My Exams

the (exam) results speak for themselves:

Did this page help you?

Lára

Author: Lára

Expertise: Biology Lead

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.