Students should be able to: Appreciate the Models & Functions of Enzyme Action
Models of enzyme action
- Scientists often use models to explain their observations from experiments
- As technology and research advances within a field new models can be developed and old ones disproven
- The lock and key model covered at GCSE was originally thought to be an accurate model of enzyme action
- It suggested that the rigid shape of the active site of the enzyme was a precise fit for the specific shape of the substrate
- New techniques have allowed scientists to discover that proteins are not rigid structures
- Experiments showed that multiple regions of an enzyme molecule moved in response to the environment
- Many of these movements were minimal but some of them were more significant
- The larger movements occurred when the substrate bound to the enzyme
- These findings led to the now widely accepted induced fit model
- Prior to binding, the substrate and active site and not completely complementary in shape
- When the substrate binds the active site alters shape and moulds around the substrate
- There is evidence to support the induced fit model:
- X-ray diffraction techniques allow for 3D pictures of molecules to be formed
- This technique was used to produce pictures of the enzyme hexokinase before and after it bound to its substrate glucose
- The images confirmed that the active site of the enzyme changed shape after the substrate bound
The lock-and-key hypothesis
The induced-fit hypothesis
Examiner Tip
Enzymes have a wide variety of functions within organisms. They catalyse both intracellular and extracellular reactions which determine the structures and functions of not only cells but the whole organism!